| Issue |
Parasite
Volume 27, 2020
|
|
|---|---|---|
| Article Number | 45 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/parasite/2020042 | |
| Published online | 25 June 2020 | |
Short Note
Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
Prévalence et identification génotypique des Cryptosporidium chez les ânes (Equus asinus asinus) en liberté et en élevage au Xinjiang, Chine
1
College of Animal Science, Tarim University, Alar, Xinjiang 843300, P.R. China
2 College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, P. R. China
3
College of Life Sciences, Tarim University, Alar, Xinjiang 843300, P.R. China
4
Department of Parasitology, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, P.R. China
* Corresponding author: qimengdz@163.com
Received:
5
December
2019
Accepted:
16
June
2020
The prevalence and zoonotic potential of Cryptosporidium in donkeys is poorly understood. Here, 680 fecal specimens were collected from 178 free-ranging and 502 farmed donkeys in Xinjiang, China. Cryptosporidium was identified using PCR amplification of the small subunit of ribosomal DNA. Cryptosporidium-positive isolates were subtyped using PCR analysis of the 60 kDa glycoprotein gene (gp60). The overall prevalence of Cryptosporidium was 2.4% (16/680), with 3.2% (16/502) in farmed donkeys and 0% (0/178) in free-ranging donkeys. Cryptosporidium hominis (n = 13), C. parvum (n = 1) and Cryptosporidium horse genotype (n = 2) were identified. The C. hominis isolates belonged to two subtypes, IkA16 (n = 9) and IkA16G1 (n = 4). The subtype of C. parvum was IIdA15G1, whereas the two Cryptosporidium horse genotype isolates were of subtype VIaA15G4. The predominance of C. hominis in donkeys suggests that these animals are infected through human contact.
Résumé
La prévalence et le potentiel zoonotique de Cryptosporidium chez les ânes sont mal connus. Dans cet article, 680 spécimens fécaux ont été prélevés de 178 ânes en liberté et 502 ânes en élevage dans le Xinjiang, en Chine. Les Cryptosporidium ont été identifiés en utilisant une amplification par PCR de la petite sous-unité de l’ADN ribosomique. Les isolats positifs pour Cryptosporidium ont été sous-typés en utilisant une analyse PCR du gène de la glycoprotéine 60 kDa (gp60). La prévalence globale de Cryptosporidium était de 2,4 % (16/680), avec 3,2 % (16/502) chez les ânes d’élevage et 0 % (0/178) chez les ânes en liberté. Cryptosporidium hominis (n = 13), C. parvum (n = 1) et Cryptosporidium génotype chevalin (n = 2) ont été identifiés. Les isolats de C. hominis appartenaient à deux sous-types, IkA16 (n = 9) et IkA16G1 (n = 4). Le sous-type de C. parvum était IIdA15G1, tandis que les deux isolats de Cryptosporidium génotype chevalin étaient du sous-type VIaA15G4. La prédominance de C. hominis chez les ânes suggère que ces animaux sont infectés par contact humain.
Key words: Cryptosporidium / Donkey / Genotyping / Zoonotic potential
© W. Wang et al., published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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