Issue |
Parasite
Volume 26, 2019
|
|
---|---|---|
Article Number | 9 | |
Number of page(s) | 11 | |
DOI | https://doi.org/10.1051/parasite/2019007 | |
Published online | 27 February 2019 |
Research Article
Histological and immunological differences between zoonotic cutaneous leishmaniasis due to Leishmania major and sporadic cutaneous leishmaniasis due to Leishmania infantum
Différences histologiques et immunologiques entre la leishmaniose cutanée zoonotique due à Leishmania major et la leishmaniose cutanée sporadique due à Leishmania infantum
1
Laboratory of Transmission, Control, and Immunobiology of Infections, Pasteur Institute of Tunis, Tunis, Tunisia
2
Laboratory of Human and Experimental Pathology, Pasteur Institute of Tunis, Tunis, Tunisia
3
Laboratory of Molecular Epidemiology and Experimental Pathology Applied to Infectious Diseases, Pasteur Institute of Tunis, Tunis, Tunisia
4
University of Tunis El Manar, Tunis, Tunisia
5
Department of Dermatology, Hospital La Rabta, Tunis, Tunisia
6
Faculté de Médecine de Tunis, Tunis, Tunisia
7
Department of Family and Community Medicine, College of Medicine and Medical Sciences, Arabian Gulf University (AGU), Manama, Bahrain
* Corresponding author: thouraya.boussoffara@pasteur.tn
Received:
25
July
2018
Accepted:
11
February
2019
Lesion features in cutaneous leishmaniasis (CL) depend on the infecting Leishmania species as well as on host immune reponse. In this study, we evaluated the histological and immunological differences between two forms of CL described in Tunisia: zoonotic cutaneous leishmaniasis (ZCL) caused by L. major and sporadic cutaneous leishmaniasis (SCL) caused by L. infantum. Histological analysis showed a mild to moderate infiltrate within ZCL lesions. In contrast, massive infiltration of the dermis was observed within SCL lesions. Contrary to ZCL, infiltrates within SCL lesions were organized and showed granuloma composed of macrophages and lymphocytes. In addition, immunohistochemical analysis showed a predominance of CD4+ T cells within both CL forms. Furthermore, expression of interferon-γ, interleukin (IL)-10, IL-8, IL-13 and monocyte chemotactic protein (MCP)-1 was evaluated using real-time quantitative polymerase chain reaction (RT-qPCR). MCP-1 and IL-10 were expressed at comparable levels in ZCL and SCL lesions. Interestingly, IL-8 mRNA levels were significantly higher in ZCL lesions compared to SCL lesions, but interferon-γ was significantly higher in SCL lesions than in ZCL lesions.
Résumé
Les caractéristiques des lésions de la leishmaniose cutanée (LC) dépendent de l’espèce de Leishmania infectante ainsi que de la réponse immunitaire de l’hôte. Dans cette étude, nous avons évalué les différences histologiques et immunologiques entre deux formes de LC décrites en Tunisie, la leishmaniose cutanée zoonotique (LCZ) causée par L. major et la leishmaniose cutanée sporadique (LCS) causée par L. infantum. L’analyse histologique a montré un infiltrat léger à modéré dans les lésions de LCZ. En revanche, une infiltration massive du derme a été observée dans les lésions de LCS. Contrairement à la LCZ, les infiltrats au sein des lésions LCS étaient organisés et montraient un granulome composé de macrophages et de lymphocytes. En outre, l’analyse immunohistochimique a montré une prédominance de cellules T CD4+ dans les deux formes de LC. De plus, l’expression de l’interféron-γ, des interleukines (IL)-10, IL-8, IL-13 et de la protéine chimiotactique monocytaire (MCP)-1 ont été évaluées en utilisant une réaction en chaîne de la polymérase quantitative en temps réel (RT-qPCR). MCP-1 et IL-10 étaient exprimés à des niveaux comparables dans les lésions LCZ et LCS. Fait intéressant, les niveaux d’ARNm d’IL-8 étaient significativement plus élevés dans les lésions LCZ que dans les lésions LCS, mais l’interféron-γ était significativement plus élevé dans les lésions LCS que dans les lésions LCZ.
Key words: Leishmania major / Leishmania infantum / lesion / cytokines / chemokines
© T. Boussoffara et al., published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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