| Issue |
Parasite
Volume 25, 2018
|
|
|---|---|---|
| Article Number | 34 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/parasite/2018038 | |
| Published online | 20 July 2018 | |
Research Article
Molecular characterization and new genotypes of Enterocytozoon bieneusi in minks (Neovison vison) in China
Caractérisation moléculaire et nouveaux génotypes d’Enterocytozoon bieneusi chez les visons (Neovison vison) en Chine
1
Marine College, Shandong University at Weihai, Weihai, Shandong Province
264209, PR China
2
General Station for Surveillance of Wildlife Diseases & Wildlife Borne Diseases, State Forestry Administration (SFA), Shenyang
110034, PR China
3
Jilin Inspection and Quarantine Technology Center, Changchun, Jilin Province
130062, PR China
4
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province
130118, PR China
* Corresponding author: messicw@163.com
Received:
12
April
2018
Accepted:
24
June
2018
Microsporidiosis is an emerging and opportunistic disease, and Enterocytozoon bieneusi is the main cause of this disease in humans. Little information is available on prevalence and genotyping of E. bieneusi in minks. We collected 559 feces samples of minks from Heilongjiang and Jilin provinces in 2017, and studied E. bieneusi prevalence by nested PCR. A total of 23 out of 559 minks (4.1%) were detected as E. bieneusi-positive, and were raised in five of the seven investigated farms. Age was the only risk factor associated with E. bieneusi prevalence in investigated minks through logistic regression analysis. Sequence analysis of the ITS gene revealed that five E. bieneusi ITS genotypes, including Peru11, EbpC, and three novel genotypes (HLJM-1, HLJM-2 and JLM-1) were present, suggesting minks may be a potential source of human microsporidiosis.
Résumé
La microsporidiose est une maladie émergente et opportuniste, et Enterocytozoon bieneusi est la principale cause de la microsporidiose humaine. Peu d’informations sur la prévalence et le génotypage d’E. bieneusi chez les visons sont disponibles. 559 échantillons d’excréments de visons provenant des provinces de Heilongjiang et de Jilin ont été collectés en 2017 et la prévalence d’E. bieneusi a été étudiée par PCR nichée. Au total, 23 (4,1%) des 559 visons ont été détectés positifs pour E. bieneusi, et provenaient de cinq des sept fermes étudiées. L’âge était le seul facteur de risque, étudiés par analyse de régression logistique, associé à la prévalence d’E. bieneusi chez les visons. L’analyse de la séquence du gène ITS a révélé que cinq génotypes ITS d’E. bieneusi, y compris Peru11, EbpC, et trois nouveaux génotypes (HLJM-1, HLJM-2 et JLM-1), étaient présents, ce qui suggère que les visons pourraient être une source potentielle de microsporidiose humaine.
Key words: Enterocytozoon bieneusi / Epidemiology / Genotyping / Minks / China
© W. Cong et al., published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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