Issue |
Parasite
Volume 25, 2018
|
|
---|---|---|
Article Number | 38 | |
Number of page(s) | 9 | |
DOI | https://doi.org/10.1051/parasite/2018035 | |
Published online | 27 July 2018 |
Research Article
Sicuophora (Syn. Wichtermania) multigranularis from Quasipaa spinosa (Anura): morphological and molecular study, with emphasis on validity of Sicuophora (Armophorea, Clevelandellida)
Sicuophora (Syn. Wichtermania) multigranularis de Quasipaa spinosa (Anura) : étude morphologique et moléculaire, avec emphase sur la validité de Sicuophora (Armophorea, Clevelandellida)
1
Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan
430072, China
2
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan
430023, China
* Corresponding author: liming@ihb.ac.cn
Received:
27
January
2018
Accepted:
18
June
2018
Morphological studies of Sicuophora (Syn. Wichtermania) multigranularis Xiao et al., 2002, from the rectum of the frog, Quasipaa spinosa, performed using silver impregnation and scanning electron microscopy, confirmed the following newly recognized features: (1) only one apical suture on the right surface; (2) two naked regions at the posterior end of both the left and the right side of the body. Phylogenetic analysis based on the SSU-rRNA gene showed that S. multigranularis is a sister to a clade comprising all other Clevelandellida, strongly supporting the validity of the genus Sicuophora. This is also the first molecular data obtained for the genus Sicuophora. Because of the lack of molecular data, it will be necessary to obtain more genetic data from the family Sicuophoridae to discuss the question of the taxonomic status of the genus Sicuophora.
Résumé
Des études morphologiques de Sicuophora (Syn. Wichtermania) multigranularis Xiao et al., 2002, du rectum de la grenouille Quasipaa spinosa, réalisées en utilisant imprégnation argentique et microscopie électronique à balayage, ont confirmé les caractéristiques suivantes, nouvellement reconnues: (1) une seule suture apicale sur la surface droite; (2) deux régions nues à l’extrémité postérieure du côté gauche et du côté droit du corps. L’analyse phylogénétique basée sur le gène SSU-rRNA a montré que S. multigranularis était le groupe-frère d’un clade comprenant tous les autres Clevelandellida, ce qui confirme fortement la validité du genre Sicuophora. Ceci est aussi la première donnée moléculaire du genre Sicuophora. En raison du manque de données moléculaires, il sera nécessaire d’obtenir plus de données génétiques de la famille Sicuophoridae pour discuter de la question du statut taxonomique du genre Sicuophora.
Key words: Clevelandellida / Endocommensal / Phylogenetic analysis / Protargol / Scanning electron microscopy / SSU-rRNA
© C. Li et al., published by EDP Sciences, 2018
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