Issue |
Parasite
Volume 25, 2018
|
|
---|---|---|
Article Number | 1 | |
Number of page(s) | 6 | |
DOI | https://doi.org/10.1051/parasite/2018002 | |
Published online | 19 January 2018 |
Short Note
Genetic characterization of three mitochondrial gene sequences of goat/sheep-derived coenurus cerebralis and cysticercus tenuicollis isolates in Inner Mongolia, China
Caractérisation génétique de trois séquences de gènes mitochondriaux d’isolats de coenurus cerebralis et de cysticercus tenuicollis de chèvre ou mouton en Mongolie intérieure, Chine
1
Department of Parasitology, Harbin Medical University China; Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity; Heilongjiang Key Laboratory for Pathogen Biology, Harbin,
Heilongjiang
150081, PR China
2
Department of MRI Scanning Room, the First Affiliated Hospital of Harbin Medical University, Harbin,
Heilongjiang
150001, PR China
* Corresponding author: liuaiqin1128@126.com
Received:
25
October
2017
Accepted:
10
January
2018
Taenia multiceps and Taenia hydatigena are widely distributed tapeworms of canids. Due to a lack of genetic information on these two parasites in China, in this study we analyzed six coenurus cerebralis and two cysticercus tenuicollis cysts from goats or sheep in Inner Mongolia, northern China by amplifying three mitochondrial genes (cox1, nad4, and cytb). Two haplotypes were obtained at each locus for either of the two Taenia cestode species, with ten nucleotide sequences being novel. The degrees of genetic variations were 1.18%, 0.61% and 0.52% for coenurus cerebralis, and 0.24%, 0.46% and 0.35% for cysticercus tenuicollis at the cox1, nad4 and cytb loci, respectively. This is the first molecular description of animal-derived metacestodes of T. multiceps and T. hydatigena in Inner Mongolia, China. Novel nucleotide sequences might reflect endemic genetic characterization of the two cestodes. The present data are useful to explore the biological and epidemiological significance of intra-specific variations within both Taenia cestodes.
Résumé
Taenia multiceps et Taenia hydatigena sont des ténias de canidés largement distribués. En raison de la quantité limitée de données génétiques sur ces deux parasites en Chine, les kystes de six coenurus cerebralis et deux cysticercus tenuicollis de chèvres ou de moutons de Mongolie intérieure, au nord de la Chine, ont été analysés en amplifiant trois gènes mitochondriaux (cox1, nad4 et cytb). Deux haplotypes ont été obtenus à chaque locus pour l’une ou l’autre des deux espèces de Taenia, avec dix séquences nucléotidiques nouvelles. Les degrés de variation génétique étaient respectivement de 1,18%, 0,61% et 0,52% pour coenurus cerebralis et de 0,24%, 0,46% et 0,35% pour cysticercus tenuicollis, aux locus cox1, nad4 et cytb. Ceci est la première description moléculaire de métacestodes d’origine animale de T. multiceps et de T. hydatigena en Mongolie intérieure, Chine. De nouvelles séquences nucléotidiques pourraient refléter des caractérisations génétiques endémiques des deux cestodes. Ces données sont utiles pour explorer la signification biologique et épidémiologique des variations intraspécifiques au sein des Taenia.
Key words: Coenurus cerebralis / cysticercus tenuicollis / sheep / goats / mitochondrial gene
© Y. Zhang et al., published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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