Issue |
Parasite
Volume 24, 2017
|
|
---|---|---|
Article Number | 11 | |
Number of page(s) | 5 | |
DOI | https://doi.org/10.1051/parasite/2017012 | |
Published online | 21 March 2017 |
Short Note
In vitro effect of two commercial anti-coccidial drugs against myxospores of Kudoa septempunctata genotype ST3 (Myxozoa, Multivalvulida)
Effet in vitro de deux médicaments anticoccidiens commerciaux contre les myxospores de Kudoa septempunctata génotype ST3 (Myxozoa, Multivalvulida)
1
School of Medicine, Jeju National University, Jeju
63243, Republic of Korea
2
Ocean and Fisheries Research Institute, Jeju Special Self-Governing Province, Pyoseon-myeon, Segwipo-si, Jeju
63629, Republic of Korea
3
Marine Applied Microbes and Aquatic Organism Disease Control Laboratory, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju
690-756, Republic of Korea
* Corresponding author: jyh4586@korea.kr
Received:
30
December
2016
Accepted:
12
March
2017
Kudoa septempunctata (Myxozoa: Multivalvulida) myxospores infect the trunk muscles of olive flounder (Paralichthys olivaceus). In this study, two popular commercially formulated anti-coccidial drugs (amprolium hydrochloride and toltrazuril) were serially diluted and incubated with purified mature Kudoa septempunctata myxospores. The viability of K. septempunctata spores was determined after a 2-day incubation followed by Hoechst 33342 and propidium iodide staining, and scanning electron microscopy. Amprolium hydrochloride significantly decreased spore viability (18% of control) at a concentration of 920 μg/mL, whereas toltrazuril showed almost no effect (83% of control). Viability of the control (untreated spores) was 90%. In vivo studies are required to confirm the efficacy of amprolium hydrochloride in fish infected with K. septempunctata myxospores on their growth and immune system performance.
Résumé
Les myxospores de Kudoa septempunctata (Myxozoa, Multivalvulida) infectent les muscles du cardeau hirame (Paralichthys olivaceus). Dans cette étude, deux médicaments anticoccidiens commercialisés populaires (chlorhydrate d’amprolium et toltrazuril) ont été dilués en série et incubés avec des myxospores mûres purifiées de K. septempunctata. La viabilité des spores de K. septempunctata a été déterminée après une incubation de 2 jours, suivie par des colorations au Hoechst 33342 et à l’iodure de propidium et microscopie électronique à balayage. Le chlorhydrate d’amprolium a significativement diminué la viabilité des spores (18 % du témoin) à une concentration de 920 μg/mL, alors que le toltrazuril n’a pratiquement pas eu d’effet (83 % du témoin). La viabilité du témoin (spores non traitées) était de 90 %. Des études in vivo sont nécessaires pour confirmer l’efficacité du chlorhydrate d’amprolium chez les poissons infectés par les myxospores de K. septempunctata sur leur croissance et leur performance immunitaire.
Key words: Anti-coccidial drug / Kudoa septempunctata / ST3 genotype / Foodborne disease / Amprolium hydrochloride / Paralichthys olivaceus
© M. Ahn et al., published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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