| Issue |
Parasite
Volume 23, 2016
|
|
|---|---|---|
| Article Number | 52 | |
| Number of page(s) | 9 | |
| DOI | https://doi.org/10.1051/parasite/2016063 | |
| Published online | 24 November 2016 | |
Research Article
Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China
Sous-typage de Cryptosporidium cuniculus et génotypage de Enterocytozoon bieneusi chez les lapins dans deux fermes de la province du Heilongjiang en Chine
1
Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang
150081, China
2
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai
200025, China
*Corresponding authors: liuaiqin1128@126.com; caojp@yahoo.com
Received:
9
August
2016
Accepted:
11
November
2016
Cryptosporidium spp. and Enterocytozoon bieneusi are two prevalent opportunistic pathogens in humans and animals. Currently, few data are available on genetic characterization of both pathogens in rabbits in China. The aim of the present study was to understand prevalence and genetic characterization of Cryptosporidium spp. and E. bieneusi in rabbits. We collected 215 fecal samples from 150 Rex rabbits and 65 New Zealand White rabbits on two different farms in Heilongjiang Province, China. Cryptosporidium spp. and E. bieneusi were tested by polymerase chain reaction (PCR) and sequencing the partial small subunit of ribosomal DNA (SSU rDNA) and the internal transcribed spacer (ITS) region of rDNA, respectively. Cryptosporidium was detected in 3.3% (5/150) of Rex rabbits and 29.2% (19/65) of New Zealand White rabbits. All the 24 Cryptosporidium isolates were identified as C. cuniculus. Enterocytozoon bieneusi was only found in 14.7% (22/150) of Rex rabbits. Five known genotypes: CHN-RD1 (n = 12), D (n = 3), Type IV (n = 2), Peru6 (n = 1), and I (n = 1), and three novel ones CHN-RR1 to CHN-RR3 (one each) were detected. By analyzing the 60-kDa glycoprotein (gp60) gene sequences of C. cuniculus isolates, three subtypes were obtained: VbA28 (n = 2), VbA29 (n = 16), and VbA32 (n = 3). All these three C. cuniculus subtypes were reported previously in humans. Four known E. bieneusi genotypes have been found to be present in humans. The three novel ones fell into zoonotic group 1. The results suggest zoonotic potential of C. cuniculus and E. bieneusi isolates in rabbits.
Résumé
Cryptosporidium spp. et Enterocytozoon bieneusi sont deux agents pathogènes opportunistes répandus chez les humains et les animaux. Actuellement, peu de données sont disponibles sur la caractérisation génétique de ces deux agents pathogènes chez les lapins en Chine. Le but de la présente étude était de comprendre la prévalence et la caractérisation génétique des Cryptosporidium spp. et E. bieneusi chez les lapins. Nous avons recueilli 215 échantillons fécaux de 150 lapins Rex et 65 lapins blancs de Nouvelle Zélande dans deux fermes différentes dans la province du Heilongjiang, en Chine. Cryptosporidium spp. et E. bieneusi ont été testés par PCR et respectivement par séquençage partiel de la petite sous-unité de l’ADN ribosomal (SSU-ADNr) et de la région ITS de l’ADNr. Cryptosporidium a été détecté dans 3.3 % (5/150) des lapins Rex et 29.2 % (19/65) des lapins blancs de Nouvelle-Zélande. Tous les 24 isolats de Cryptosporidium ont été identifiés comme C. cuniculus. Enterocytozoon bieneusi n’a été trouvé que dans 14.7 % (22/150) des lapins Rex. Cinq génotypes connus : CHN-RD1 (n = 12), D (n = 3), Type IV (n = 2), Peru6 (n = 1) et I (n = 1), et trois nouveaux, CHN-RR1 à CHN-RR3 (un de chaque) ont été détectés. En analysant les séquences du gène de la glycoprotéine de 60 kDa (gp60) des isolats de C. cuniculus, trois sous-types ont été obtenus: VbA28 (n = 2), VbA29 (n = 16) et VBA32 (n = 3). Ces trois sous-types de C. cuniculus ont été rapportés auparavant chez l’homme. Quatre génotypes d’E. bieneusi connus ont été rapportés chez les humains. Les trois nouveaux appartiennent au groupe zoonotique 1. Les résultats suggèrent un potentiel zoonotique des isolats de C. cuniculus et E. bieneusi des lapins.
Key words: rabbits / Cryptosporidium / Enterocytozoon bieneusi / ITS region / SSU rDNA / gp60 gene
© Z. Yang et al., published by EDP Sciences, 2016
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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