Issue |
Parasite
Volume 21, 2014
|
|
---|---|---|
Article Number | 6 | |
Number of page(s) | 3 | |
DOI | https://doi.org/10.1051/parasite/2014007 | |
Published online | 19 February 2014 |
Research Article
Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy
Caractérisation moléculaire de Toxoplasma gondii Type II dans les avortements d’ovins en Sardaigne, Italie
Istituto Zooprofilattico Sperimentale della Sardegna, Via Duca degli Abruzzi, 8, 07100
Sassari, Italy
* Corresponding author: giovanna.masala@izs-sardegna.it
Received:
10
December
2013
Accepted:
10
February
2014
During 2009–2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5′-SAG2, 3′-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.
Résumé
Pendant la période 2009–2010, 161 échantillons de tissus (142 placentas, 16 cervelles et 3 foies) provenant de fœtus ovins avortés de Sardaigne, Italie, ont été testés pour la toxoplasmose. Les organes trouvés positifs par PCR visant la région ITS1 de Toxoplasma gondii ont été amplifiés avec 11 marqueurs génétiques (SAG1, 5′-SAG2, 3′-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 et Apico) et ensuite soumis à PCR/RFLP pour génotypage. De l’ADN de T. gondii a été trouvé dans 5 placentas, 14 cervelles et 2 foies par les analyses PCR et tous les isolats avaient des allèles de Type II pour les 11 loci avec tous les 11 marqueurs. Les résultats indiquent que T. gondii de Type II est associé avec les avortements ovins.
Key words: Toxoplasma gondii / Genetic characterization / Sheep / Abortion
© G. Chessa et al., published by EDP Sciences, 2014
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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