Issue |
Parasite
Volume 21, 2014
|
|
---|---|---|
Article Number | 36 | |
Number of page(s) | 5 | |
DOI | https://doi.org/10.1051/parasite/2014035 | |
Published online | 31 July 2014 |
Research Article
Cryptosporidiosis in broiler chickens in Zhejiang Province, China: molecular characterization of oocysts detected in fecal samples
Cryptosporidiose chez les poulets de chair dans la province du Zhejiang, Chine : caractérisation moléculaire des oocystes détectés dans les échantillons fécaux
1
Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang
310058, PR China
2
Hangzhou College of Commerce, Zhejiang Gongshang University, Hangzhou, Zhejiang
310012, PR China
3
Faculty of Life Science and Technology, Ningbo University, Ningbo, Zhejiang
315211, PR China
* Corresponding author: afdu@zju.edu.cn
Received:
8
December
2013
Accepted:
26
June
2014
Cryptosporidium is one of the most important parasites in poultry, and this pathogen can infect more than 30 avian species. The present study investigated the infection rate of Cryptosporidium among broiler chicken flocks. A total of 385 fecal samples from broiler chickens in 7 regions of Zhejiang Province collected from November 2010 to January 2012 were examined by microscopy. Thirty-eight (10%) samples were positive for Cryptosporidium infection, and 3 genotypes (Cryptosporidium baileyi, Cryptosporidium meleagridis, and avian genotype II) were identified by PCR and sequencing. A phylogenetic tree of the isolates was analyzed. These results suggest that cryptosporidiosis is widespread in poultry in Zhejiang Province, and is a potential threat to public health as well as the economy. This is the first report about the infection rate and molecular characterization of Cryptosporidium in broiler chickens in Zhejiang.
Résumé
Cryptosporidium est l’un des parasites les plus importants de la volaille, et cet agent pathogène peut infecter plus de 30 espèces aviaires. La présente étude a examiné le taux d’infection de Cryptosporidium chez les poulets de chair. Un total de 385 échantillons fécaux de poulets de chair dans 7 régions de la province du Zhejiang, recueillis de novembre 2010 à janvier 2012, ont été examinés par microscopie. Trente-huit (10 %) étaient positifs pour l’infection à Cryptosporidium, et 3 génotypes (Cryptosporidium baileyi, Cryptosporidium meleagridis, génotype aviaire II) ont été identifiés par PCR et séquençage. Un arbre phylogénétique des isolats a été analysé. Ces résultats suggèrent que la cryptosporidiose est répandue chez les volailles dans la province du Zhejiang, et est une menace potentielle pour la santé publique ainsi que l’économie. Ceci est le premier rapport sur le taux d’infection et la caractérisation moléculaire de Cryptosporidium dans les poulets de chair dans le Zhejiang.
Key words: Cryptosporidium / Infection rate / China / Chicken / Epidemiology
© L. Wang et al., published by EDP Sciences, 2014
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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