Issue |
Parasite
Volume 16, Number 3, September 2009
|
|
---|---|---|
Page(s) | 235 - 239 | |
DOI | https://doi.org/10.1051/parasite/2009163235 | |
Published online | 15 September 2009 |
Note de recherche
Detection of Cryptosporidium oocysts in green mussels (Perna viridis) from shell-fish markets of Thailand
Détection d’oocystes de Cryptosporidium chez la moule verte (Perna viridis) sur des marchés de poissons en Thailande
1
Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand
2
Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
3
University of Cologne, Medical School, Anatomy II, Medical and Molecular Parasitology Laboratory, Cologne, Germany
4
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
* Correspondence: Mayuna Srisuphanunt. E-mail: mayunaph@yahoo.com; phmayuna@staff2.mahidol.ac.th
Received:
25
March
2008
Accepted:
25
March
2009
Mussels filter large volumes of water and can concentrate pathogenic organisms, which may act as potential vehicles of transmission to the consumer. A survey study was carried out to investigate the presence of Cryptosporidium protozoan parasites in green mussels (Perna viridis), the smussles pecies most destined for consumption in Thailand. In total, 56 samples were examined from Bangkok (n = 24) and Samut Prakan (n = 32) a wholesale shell-fish markets located at the mouth of the Chao Phraya River. The market for green mussels was closed to the mussel culture placed along the coastal line and this localization may have significant economical impact if the mussels’ cultures are found contaminated. Cryptosporidium spp. oocysts were detected by the immunofluorescence antibody method (IFA) in 12.5% of the samples examined. The detection of Cryptosporidium oocysts in green mussels’ population of Samut Prakan was higher (15.6%) than in Bangkok market (8.3%). These differences in positive samples from the two locations may be caused by physical, ecological and anthropogenic conditions. This could relay to different contamination levels of marine water by Cryptosporidium oocysts and consequently to contamination of harvested shellfish populations. The results demonstrate that the Cryptosporidium spp. oocysts were found indigenous in mussels from the coastal line of Thailand, indicating that mussels may act as a reservoir of Cryptosporidium foodborne infections for humans.
Résumé
Les moules filtrent un important volume d’eau et peuvent héberger des pathogènes potentiellement transmissibles aux consommateurs. Une étude a été menée afin de détecter la presence de Cryptosporidium, protozoaires parasites de la moule verte (Perna viridis), espèce la plus consommée en Thaïlande. 56 prélèvements ont été réalisés à Bangkok (n = 24) et à Samut Prakan (n = 32), un marché de vente en gros de coquillages et poissons, situé à l’embouchure de la rivière Chao Phraya, sachant qu’une contamination des moules aurait un important impact économique sur ce marché qui est proche des parcs d’élevage situés le long de la côte. Des oocystes de Cryptosporidium spp. ont été détectés par immunofluorescence (IFA) chez 12,5 % des prélèvements, avec un pourcentage d’infestation plus élevé à Samut Prakan (15,6 %) qu’à Bangkok (8,3 %) qui pourrait s’expliquer par des conditions physiques, écologiques et anthropologiques différentes. Les résultats montrent principalement que des oocystes de Cryptosporidium spp. sont présents dans les moules de la côte thaïlandaise, lesquelles peuvent être considérées comme un réservoir de cryptosporidiose humaine d’origine alimentaire.
Key words: Cryptosporidium / green mussels / Thailand
Mots clés : Cryptosporidium / moule verte / Thaïlande
© PRINCEPS Editions, Paris, 2009, transferred to Société Française de Parasitologie
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