Issue |
Parasite
Volume 13, Number 1, March 2006
|
|
---|---|---|
Page(s) | 71 - 74 | |
DOI | https://doi.org/10.1051/parasite/2006131071 | |
Published online | 15 March 2006 |
Note de recherche
Comparative evaluation of antibody positive titer by ELISA and IFA in Theileria annulata vaccinated cattle in Iran
Évaluation comparative par ELISA et IFA des taux d’anticorps du bétail vacciné par Theileria annulata en Iran
1
Protozoology Department, Razi Vaccine & Serum Research Institute,P.O. Box 11365/1558, Tehran, Iran
2
Biotechnology Department, Razi Vaccine & Serum Research Institute,P.O. Box 11365/1558, Tehran, Iran
* Correspondence: R. Hashemi-Fesharki. E-mail: rezahf2001@yahoo.co.uk
Received:
30
September
2004
Accepted:
3
November
2005
An enzyme linked immunosorbent assay (ELISA) was used to evaluate antibody positive titer in vaccinated and non-vaccinated cattle using schizont infected myeloid cells as an antigen. The result was compared with indirect fluorescent antibody level in the same animals. For this study 116 milking cows, 95 vaccinated and 21 non-vaccinated, were bleeded in order to prepare sera. They were tested with both ELISA and IFA tests. 94 sera had positive antibody titer and 22 sera were negative through ELISA test but, with IFA test, only 89 sera showed positive antibody titer and 27 were negative. Thereby, it was concluded that the sensitivity and specificity of ELISA test in comparison with IFA test was 95.5 % and 66.6 % respectively. This study generally indicated that ELISA could be an effective test for seroepidemiological investigations of bovine tropical theileriosis, and it is considered to be valid as an additional test to distinguish the vaccinated from the non vaccinated cattle in order to schedule vaccination programs.
Résumé
Un test ELISA a été utilisé afin d’évaluer les taux d’anticorps du bétail vacciné ou non, en ayant recours en tant qu’antigène à des cellules myéloïdes infectées par des schizontes. Les résultats ont été comparés à ceux obtenus par immunofluorescence indirecte chez les mêmes animaux. Les sérums de 116 vaches laitières, 95 vaccinées et 21 non vaccinées, ont été examinés. Ils ont été testés par ELISA et IFA : 94 sérums avaient des taux d’anticorps positifs et 22 négatifs en ELISA, pour 89 positifs et 27 négatifs en IFA. Ainsi, les sensitivités et spécificités des tests en ELISA comparées à celles obtenues en IFA ont été de 95,5 et 66,6 % respectivement. Cette étude indique de façon générale que l’ELISA pourrait être un bon test séro-épidémiologique de détection de la theileriose bovine tropicale, et de distinction des animaux vaccinés et non vaccinés pour l’élaboration de programmes de vaccinations.
Key words: Theileria annulata / ELISA / IFA / vaccination
Mots clés : Theileria annulata / ELISA / IFA / vaccination
© PRINCEPS Editions, Paris, 2006, transferred to Société Française de Parasitologie
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.