Issue |
Parasite
Volume 11, Number 1, March 2004
|
|
---|---|---|
Page(s) | 103 - 106 | |
DOI | https://doi.org/10.1051/parasite/2004111103 | |
Published online | 25 August 2014 |
Note de recherche
Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation
Trypanosoma vivax : un protocole simple pour la culture in vivo, l’isolement et la cryoconservation
Institute of Tropical Medicine, Department of Parasitology, Nationalestraat 155, B-2000 Antwerp, Belgium.
* Correspondence : Dr Momar Ndao, present address: National Reference Centre for Parasitology (NRCP), Montreal General Hospital Research Institute R3-107, 1625 Pine Ave West, Montreal, QC, H3G 1A4, Canada. Tel.:1-514-934-8347 - Fax : 1-514-934-8261. E-mail: momar.ndao@staff.mcgill.ca
Received:
25
April
2003
Accepted:
8
September
2004
A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of this simple procedure for trypanosome purification were about six times higher than those obtained with the conventional anion-exchange columns. Cryopreservation of trypanosomes using glycerol yielded 90 % viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35 %.
Résumé
Un clone de Trypanosoma vivax adapté aux rongeurs a été utilisé pour infecter des souris et des rats traités au cyclophosphamide. Du sang frais contenant des trypanosomes a été centrifugé dans un gradient de densité de trois solutions de Percoll respectivement 1,07, 1,06, 1,05 g/ml et soigneusement déposées l'une sur l’autre. Les rendements de ce procédé simple pour la purification des trypanosomes étaient environ six fois plus élevés que ceux obtenus avec les colonnes conventionnelles d'échange anionique. Après cryoconservation des trypanosomes en utilisant le glycérol, le pourcentage de parasites viables était de 90 %, tandis qu'en utilisant le diméthylsulfoxyde, la viabilité était seulement de 35 %.
Key words: Trypanosoma vivax / cyclophosphamide / isolation / cryopreservation / Percoll / rodents
Mots clés : Trypanosoma vivax / cyclophosphamide / isolation / cryopreservation / Percoll / rongeur
© PRINCEPS Editions, Paris, 2004, transferred to Société Française de Parasitologie
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.