Issue |
Parasite
Volume 10, Number 2, June 2003
|
|
---|---|---|
Page(s) | 141 - 145 | |
DOI | https://doi.org/10.1051/parasite/2003102141 | |
Published online | 27 August 2014 |
Mémoire
The determination of immune reactive proteins in Cysticercus tenuicollis cyst fluids by SDS-PAGE and Western blotting in sheep
Recherche des protéines immunoréactives dans le liquide de kyste de Cysticercus tenuicollis par SDS-PAGE et Western Blot chez le mouton
1
Department of Parasitology, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
2
Department of Helminthology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey.
* Correspondence : Dr Murat Kara. Tel. : 90 474 2426874 - Fax : 90 474 242 6853. E-mail : muratkara96@yahoo.com
Received:
7
September
2002
Accepted:
23
January
2003
Cysticercus tenuicollis, the larva of Taenia hydatigena, is commonly seen in sheep and goats slaughtered in Turkey. In this study, the protein bands were revealed in C. tenuicollis cyst fluid antigen by using SDS-PAGE and Western blotting, and immune reactive bands were determined. Ten positive and 10 negative sera for C. tenuicollis from sheep and one non-infected sheep serum were tested in this experiment. According to the results, there was only one protein band determined to be immune reactive, which was 36 kDa.
Résumé
Cysticercus tenuicollis, la larve de Taenia hydatigena, est généralement vu chez les moutons et les chèvres abattues en Turquie. Dans cette étude, des bandes de protéines ont été observées dans le liquide antigénique de kystes de C. tenuicollis par SDS-PAGE et Western Blot, et des bandes immunoréactives ont été recherchées. Dix sérums de moutons positifs et 10 négatifs vis-à-vis de C. tenuicollis et un sérum de mouton non infecté ont été examinés dans cette étude. Selon les résultats, une seule bande de protéines de 36 kDa a été déterminée comme étant immunoréactive.
Key words: Cysticercus tenuicollis / sheep / SDS-PAGE / Western blotting
Mots clés : Cysticercus tenuicollis / moutons / SDS-PAGE / Western Blot
© PRINCEPS Editions, Paris, 2003, transferred to Société Française de Parasitologie
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