Issue |
Parasite
Volume 8, Number 3, September 2001
|
|
---|---|---|
Page(s) | 251 - 254 | |
DOI | https://doi.org/10.1051/parasite/2001083251 | |
Published online | 02 September 2014 |
Note de recherche
Ultrastructural studies of sarcocystis cruzi (Hasselmann,1926) Wenyon, 1926 infection in cattle (Bos Taurus): Philippine cases
Étude ultrastructurale de l’infestation du bovin (Bos taurus) par Sarcocystis Cruzi (Hasselmann, 1926) Wenyon, 1926 : un cas philippin
1
Biology Department, College of Science, De La Salle University- Manila, 2401 Taft Avenue, Manila, Philippines.
2
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro 080-8555, Japan.
* Correspondence: Florencia G. Claveria, Biology Department, College of Science, De La Salle University-Manila, Taft Avenue, Manila, Philippine. Telefax:632-524-0451 E-mail: COSFGC@mail.dlsu.edu.ph
Received:
21
August
2000
Accepted:
21
June
2001
This paper documents the first report of Sarcocysli s cruzi infection in domesticated cattle (Bos taurus) in the Philippines. Fusiformshaped microscopic sarcocysts (183-578 μm long and 20-98 μm wide) with distinct septae were found in the skeletal, striated and heart muscle. The sarcocyst wall or parasitophorous vacuolar membrane, 1.37-2.75 μm thick consisted of closely-packed villar protrusions 80-400 nm in dm. Middle and distal segments of VP were bent approximately 90 degrees parallel to the cyst wall surface. The villar core lacked microtubules, and at some points, the distal ends of the VP collectively formed conical tufts. Primary cyst wall had numerous 70-100 nm bubble-like undulations, and the ground substance was 0.25-0.5 μm in thickness. The ultrastructure of S. cruzi cyst wall typifies the Type 7 sarcocyst wall, and bears close similarities with the Philippine and the Vietnam strain of bubaline Sarcocystis levinei.
Résumé
Il s'agit de la première observation de l'infestation du bovin domestique (Bos taurus) par Sarcocystis cruzi aux Philippines. Des sarcocystes fusiformes (183-578 μm de long sur 20-98 μm de large) avec un septum distinct ont été observés au niveau des muscles striés squelettiques et cardiaque. La paroi du sarcocyste ou membrane vacuolaire parasitophore, épaisse de 1,37- 2,75 μm présente en un amas de protrusions villositaires (PV) de 80-400 nm de diamètre. Les segments moyen et distal des PV sont fléchis d'environ 90° par rapport à la surface de la paroi. Le corps de la villosité manque de microtubule et, en certains endroits, les extrémités distales des PV forment des huppes coniques. La paroi des cystes présente des ondulations qui ressemblent à des bulles de 70-100 nm, et la membrane basale est épaisse de 0,25-0,50 μm. L'ultrastructure de la paroi de S. cruzi est de même type que celle de la paroi du sarcocyste de Type 7, et présente d'étroites similitudes avec les souches de Sarcocystis levinei observées chez le bubale au Philippines et au Vietnam.
Key words: Sarcocystis cruzi / ultrastructure / Philippine cases / Bos taurus
Mots clés : Sarcocystis cruzi / cas philippin / ultrastructure / Bos taurus
© PRINCEPS Editions, Paris, 2001, transferred to Société Française de Parasitologie
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