Volume 8, june 2001Xth International Conference on Trichinellosis (20-24 august 2000)
|Page(s)||S30 - S33|
|Published online||17 September 2014|
Xth ICT, August 2000
Estimating the genetic divergence and identification of three trichinella species by isoenzyme analysis
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovakia.
2 Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Ridebanevej 3, DK-1870 Frederiksberg C, Denmark.
3 Department of Infectious Diseases, Lithuanian Veterinary Academy, Tilzes 18, 3022 Kaunas, Lithuania.
* Correspondence : Viliam Šnábel. Fax : +421 95 6334455 - Tel. : +421 95 6331414 E-mail : email@example.com
Isoenzyme-based approach was applied to compare Trichinella spiralis, T. britovi and T. pseudospiralis species. Among 13 enzyme systems examined, esterase (EST), malic enzyme (ME) and phosphoglucomutase (PGM) have been found as fully diagnostic, with no common allele in species studied. Adenosine deaminase (ADA), adenylate kinase (AK), hexokinase (HK), peptidase leucyl-alanine (PEP-C) and fructose-bis-phosphatase (FBP) have been capable of distinguishing the two species from resulting profiles. In addition, ADA, AK and PGM displayed the enzyme expression in the lowest amounts of muscle larvae in systems tested ( 100 larvae/100 μl of extracts). Based on allozyme data, T. pseudospiralis has been found as the most distinct species within the group of taxa. Only a subtle genetic variability was recorded for T. pseudospiralisin which solely phosphoglucomutase exhibited variant patterns. In addition to the study of reference isolates, T. spiralis from lowland fox in Eastern Slovakia has been evidenced by use of genetic markers. This finding has proved that T. britovi is not the exclusive species parasitizing in the sylvatic ecosystem of the Slovak region.
Key words: Trichinella / isoenzymes / diagnosis / isoelectrofocusing
© PRINCEPS Editions, Paris, 2001, transferred to Société Française de Parasitologie
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.