Volume 8, june 2001Xth International Conference on Trichinellosis (20-24 august 2000)
|Page(s)||S260 - S262|
|Published online||18 September 2014|
Xth ICT, August 2000
Immunodiagnosis of trichinella infection in the horse
Institute for the Application of Nuclear Energy (INEP), Banatska 31b, 11080 Belgrade, Yugoslavia.
2 Istituto Superiore di Sanità (ISS), viale Regina Elena 299, 00161 Rome, Italy.
3 A.R.T.E. International SRL, Pascal Cristian 33, 77713 Bucharest, Romania.
4 USDA, Parasite Biology and Epidemiology Laboratory, Beltsville. MD 20705, USA.
* Correspondence : Sofronic-Milosavljevic Ljiljana. UMR 956 BIPAR, INRA-AFSSA-ENVA, 22, rue Pierre Curie, 94700 Maisons-Alfort, France. Tel. : +33 1 49 77 13 28 - Fax : +33 1 49 77 13 16. E-mail : email@example.com
From 1998 to 2000, 5,267 horse sera were collected from several Trichinella regions in Romania. Sera were initially screened in laboratories in Romania, Serbia and Italy with an ELISA and a Western blot (Wb) using an excretory/secretory (ES) antigen and several conjugates (protein A, protein G, and sheep or goat anti-horse). Differences in serology results were obtained among the different conjugates and also between ELISA and Wb. Depending on the test used, specific antibodies were found at a prevalence rate of 3-6 % of horses. Serum samples classified as positive were tested again by ELISA using a synthetic tyvelose glycan-BSA antigen, in Italy. All serum samples tested using this antigen were negative; in contrast, serum samples from experimentally infected horses were positive with the glycan antigen. The negative results obtained with the glycan antigen are consistent with the low prevalence of horse trichinellosis reported in the literature. Based on these results, further studies are needed to validate immunodiagnostic tests to detect Trichinella infection in horses.
Key words: horse / trichinellosis / epidemiology / serology / Romania
© PRINCEPS Editions, Paris, 2001, transferred to Société Française de Parasitologie
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