| Issue |
Parasite
Volume 8, june 2001
Xth International Conference on Trichinellosis (20-24 august 2000)
|
|
|---|---|---|
| Page(s) | S257 - S259 | |
| DOI | https://doi.org/10.1051/parasite/200108s2257 | |
| Published online | 17 September 2014 | |
Xth ICT, August 2000
Detection of Trichinella infection in slaughter horses by artificial digestion, ELISA and PCR
1
UIMEIP, Centro Médico Nacional Siglo XXI,
2
CENID-Microbiología, INIFAP, SAGAR, Km 15.5 Carretera México-Toluca, México, D.F.
3
Dpto. de Genética y Biologia Molecular CINVESTAV, IPN, México, D.F.
* Correspondence : C. Arriaga. Tel.: 52-5-570-310 ext. 30 - Fax : Fax: 52-5-570-4073 E-mail : camila@micro.inifap.conacyt.rnx
In this study we compared the sensitivity of molecular, serologic and parasitologic methods for diagnosis of equine trichinellosis in two abattoirs, one rural and one federal inspection type. Diaphragm muscle samples were obtained from 170 slaughter horses and examined by artificial digestion and PCR. Serum samples from these horses were also analyzed by ELISA. No Trichinella muscle larvae were detected by artificial digestion. However, specific antibodies against Trichinella were detected in 17 % and 7 % of the serum samples examined from the rural and the federal abattoirs respectively. By PCR, 15 % and 2 % of the samples from these two abattoirs were Trichinella positive.
Key words: trichinellosis / horse / PCR
© PRINCEPS Editions, Paris, 2001, transferred to Société Française de Parasitologie
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