Volume 8, june 2001Xth International Conference on Trichinellosis (20-24 august 2000)
|Page(s)||S141 - S143|
|Published online||17 September 2014|
Xth ICT, August 2000
The use of a synthetic antigen for the serological diagnosis of human trichinellosis
Dipartimento di Patologia Sperimentale, B.M.I.E., Università di Pisa, Via Roma 55, 56126 Pisa, Italy.
2 Dipartimento di Scienze Biopatologiche Veterinarie, Università di Perugia, Via S. Costanzo 4, 06100 Perugia, Italy.
3 Heska Corporation, 1825 Sharp Point Drive, Fort Collins, Colorado, USA.
* Correspondence : F. Bruschi. Tél.: +39(050)554851 - Fax : +39(050)554929. E-mail : firstname.lastname@example.org
Hosts infected with Trichinella produce antibodies specific for an epitope common to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed, which was specific and sensitive in swine trichinellosis. We aimed to verify the specificity and sensitivity of this immunodiagnostic test in human trichinellosis. 15 sera from normal subjects, 12 from patients with other parasitic diseases and 50 from trichinellosis patients were tested. Indirect enzyme linked immunosorbent assay (ELISA) for specific IgG and an amplified ELISA for specific IgE were performed using β-tyvelose-GalNAc-bovine serum albumin (BSA) disaccharide conjugate or T. spiralis muscle larvae excretory/secretory (E/S) products, as antigens. Neither control sera nor other parasitic infection sera resulted positive both for IgG and IgE when synthetic or E/S antigens were used. In trichinellosis patient sera, specific IgG were present in 100 % of cases, irrespective of the antigen used, but whereas specific IgE were detected in 78 % using E/S antigens, a 100% positivity rate was obtained, using the β-tyvelose- BSA conjugate.
Key words: β-tyvelose / human trichinellosis / lgG / lgE / diagnosis
© PRINCEPS Editions, Paris, 2001, transferred to Société Française de Parasitologie
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