Volume 8, june 2001Xth International Conference on Trichinellosis (20-24 august 2000)
|Page(s)||S123 - S125|
|Published online||17 September 2014|
Xth ICT, August 2000
Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
Zoonosis and Biotechnology Departments, INDRE, Ministry of Health
2 Biotechnology Departments, INDRE, Ministry of Health.
3 Faculty of Medicine, UNAM.
* Correspondence : J.-L. De La Rosa, Carpio 470, Mexico D. F. 11340. Mexico. Tel.: (525)3414953 ext. 224. - Fax : (525)3413264. E-mail : email@example.com.
Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae which recognize both somatic and metabolic antigens from adult and muscular larvae (ML). We used two lgG3 MAbs (4B1, 4B2) and two lgG2a (2D3, 2D4) to detect antigens during experimental infection. All MAbs detect fecal antigens starting the second to third week post infection (wpi), although it was less clear with MAbs 4B1 and 4B2, while circulating antigens were detected from third to fourth wpi. Thus, the recognition of shared antigens among T. spiralis developmental stages can be used for early diagnosis of trichinellosis.
Key words: Trichinella / antigens / monoclonal-antibody / diagnosis / new born larva
© PRINCEPS Editions, Paris, 2001, transferred to Société Française de Parasitologie
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