Issue |
Parasite
Volume 5, Number 1, March 1998
|
|
---|---|---|
Page(s) | 27 - 36 | |
DOI | https://doi.org/10.1051/parasite/1998051027 | |
Published online | 23 September 2014 |
Mémoire
The in vitro effects of crystal violet on the pathogenic haemoflagellate Cryptobia salmositica Katz, 1951 (Sarcomastigophora : Kinetoplastida)
Les effets in vitro du cristal violet sur l'hémoflagellé Cryptobia salmositica Katz, 1951 (Sarcomastigophora : Kineto-Plastida)
Department of Zoology, University of Guelph, Guelph, Ontario, Canada, NIG 2W1.
* Correspondence to : P.T.K. Woo. Fax : (519) 767-1656 - Tel. : (519) 824-4120 E-mail : pwoo@uoguelph.ca
Received:
22
May
1997
Accepted:
16
September
1997
Crystal violet does not inhibit in vitro multiplication of a nonpathogenic strain of Cryptobia salmositica at low concentrations (0.01 μM and 0.001 μM) but multiplication is inhibited at higher concentrations (≥ 0.05 μM). In contrast, the pathogenic strain of C. salmositica does not multiply in vitro when incubated with crystal violet (0.001 μM, 0.01 μM and 0.05 μM). The infectivity of the pathogenic strain is significantly reduced after in vitro exposure to crystal violet. Crystal violet lyses C. salmositica (100.0 μM ) and causes lesions on mitochondrial and nuclear membranes of the parasite. Pathogenic strains of Cryptobia salmositica and C. bullocki are more susceptible to lysis after in vitro exposure to crystal violet than are nonpathogenic strains of Cryptobia salmositica and C. catostomi
Résumé
Le cristal violet, à de faibles concentrations 10.01 μM et 0.001 μM), n'empêche pas la multiplication in vitro de la souche non pathogène de Cryptobia salmositica. Par contre, la souche pathogène de C. salmositica ne se multiplie pas in vitro lorsqu'elle est incubée avec du cristal violet (0.001 μM, 0.01 μM et 0.05 μM). L'infectivité de la souche pathogène est réduite significativement après une exposition au cristal violet. Le cristal violet lyse C. salmositica (100.0 μM) et cause des lésions aux membranes mitochondriales et nucléaires du parasite. Les souches pathogènes de Cryptobia salmositica et C. bullocki sont plus susceptibles à la lyse après une exposition au cristal violet que les souches non-pathogènes de Cryptobia salmositica et C. catostomi.
Key words: crystal violet / toxicity / Cryptobia
Mots clés : cristal violet / toxicité / Cryptobia
© PRINCEPS Editions, Paris, 1998, transferred to Société Française de Parasitologie
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