Issue |
Parasite
Volume 4, Number 1, March 1997
|
|
---|---|---|
Page(s) | 27 - 35 | |
DOI | https://doi.org/10.1051/parasite/1997041027 | |
Published online | 24 September 2014 |
Mémoire
Lectins of Trichobilharzia szidati cercariae
Lectines de cercaires de Trichobilharzia szidati
1
Department of Parasitology, Charles University, Prague 2, Czech Republic.
2
Institute of Parasitology, Academy of Sciences of the Czech Republic, České Budĕjovice, Czech Republic.
3
Department of Biochemistry. Charles University, Prague 2, Czech Republic.
* Correspondence : Petr Horák, Department of Parasitology, Charles University. Viničná 7, 128 44 Prague 2. Czech Republic. Tel :++420.2.21953196 - Fax : ++420.2.299713 E-mail : horak@mail.natur.curi.cz
Received:
19
August
1996
Accepted:
25
November
1996
Cercariae of Trichobilharzia szidati were examined for the presence of endogenous lectins. The haemagglutination caused by the cercarial homogenate was inhibited by glycoconjugates (heparin, hyaluronic acid, lipopolysaccharide, bovine submaxillar mucin, thyroglobulin) and saccharides (lactulose, laminarin, D-galacturonic acid). Ligand blotting with laminarin-conjugate revealed the existence of one laminarin-binding protein in the sample. This protein migrates mostly as a double-band under non-reducing conditions (48-52 kDa), and as a single-band under reducing conditions (54-56 kDa). Identical bands were recognized by specific mouse antibodies raised against agglutinins bound on mouse erythrocytes. Labeled laminarin and/or heparin reacted with postacetabular glands on histological sections. Similarly, the binding of Lotus tetragonolobus lectin as a glycoprotein ligand supported the finding that the cercarial lectin is localized in postacetabular glands. Moreover, there is an indication that a lectin is present on the cercarial surface. In agreement with affinity fluorescence, mouse antibodies to the cercarial haemagglutinins recognized the postacetabular penetration glands and the surface of cercariae.
Résumé
Des lectines endogènes ont été recherchées dans des extraits de cercaires de Trichobilharzia szidati. L'hémagglutination provoquée par Thomogénat de cercaires est inhibée par des substances glycoconjuguées (héparine, acide hyaluronique, lipopolysaccharide, mucine de sous-maxillaire bovine, thyroglobulinej et des saccharides (lactulose, laminarine, acide D-galacturoniquej. La réaction de floculation avec un conjugué de laminarine révèle l'existence d'une protéine de liaison à la laminarine dans l'échantillon. Cette protéine migre principalement : en deux bandes en conditions non réduites (48-52 kDa), et en une seule bande SDS-PACE en conditions réduites (54-56 kDa). Des bandes identiques sont reconnues par des anticorps de souris spécifiques dirigés contre des agglutinines liées à des érythrocytes de souris. La laminarine et/ou l'héparine réagit avec les glandes post-acétabulaires sur des coupes histologiques. De même la liaison de la lectine de Lotus tetragonolobus conforte la localisation de la lectine de cercaire dans les glandes post-acétabulaires. De plus, une lectine pourrait être située à la surface des cercaires. En immunofluorescence, les anticorps de souris dirigés contre les hémagglutinines de cercaire reconnaissent.
Key words: Trichobilharzia szidati / schistosomes / cercaria / lectin / agglutinin
Mots clés : Trichobilharzia szidati / schistosomes / cercaire / lectine / agglutinine
© PRINCEPS Editions, Paris, 1997, transferred to Société Française de Parasitologie
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