Volume 1, Number 1S, March 19943rd CEC Filariasis Network Meeting (Lisbon -IHMT, 4-7 September 1993)
|Page(s)||S55 - S57|
|Published online||01 October 2014|
Isolation of new markers to detect genetic variation in Onchocerca volvulus
Laboratoire d'Epidémiologie de Maladies à Vecteurs, Centre ORSTOM de Montpellier, 911 av. Agropolis, BP 5045, 34032 Montpellier, France.
2 Laboratoire Rétrovirus-Parasites, Centre ORSTOM de Montpellier, 911 av. Agropolis, BP 5045, 34032 Montpellier, France.
Newly developed techniques (RADP: Random Amplified Polymorphic DNA and microsatellite DNA sequences) were used to study genetic variation in Onchocerca volvulus. RAPD technique, derived from the Polymerase Chain Reaction (PCR), allow clear distinction between the different species of the genus Onchocerca.
Microsatellite DNA has been shown to be useful as polymorphic markers for populations and even for Individuals. Short repeated sequences (CA repeats) have been isolated, and a PCR assay using such microsatellite DNA sequences to generate polymorphisms is currently being experimented.
Key words: Onchocerca volvulus / genetic variability / RAPD / microsatellite / PCR
© PRINCEPS Editions, Paris, 1994, transferred to Société Française de Parasitologie
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