Issue |
Ann. Parasitol. Hum. Comp.
Volume 68, Number 4, 1993
|
|
---|---|---|
Page(s) | 163 - 168 | |
DOI | https://doi.org/10.1051/parasite/1993684163 | |
Published online | 12 August 2016 |
Mémoire
Laboratory and field studies on Herpetosoma Trypanosomes from Portugal1
Études expérimentales de Trypanosomes (Herpetosoma) de petits mammifères portugais
1
Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 96, 13000 Lisboa., Portugal.
2
Department of Biological Sciences, University of Salford, Salford M5 4WT (Present address: School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK)., England.
Accepted: 1 February 1993
Several small mammals were trapped in the Arrabida region (Portugal) and checked for the presence of trypanosomes which were found in 33 of the 197 (11.1 %) Mus spretus and in 9 of the 29 (31 %) Crocidura russula observed. To our knowledge, this was the first time that trypanosomes were isolated from these mammals species. In the liver of one dead C. russula was observed different parasite forms. The studies of infectivity to experimental rodents, analyses of the DNA buoyant density and the isoenzymatic profils, show that trypanosomes isolates from M. spretus were identical to Trypanosoma musculi isolates from Mus musculus. However the isolates from C. russula, although related to the isolates from murine rodents, were clearely separated from these and close to Trypanosoma microti. These findings may allow further studies on the detection of their vectors and on the study of trypanosome reproduction.
Résumé
Dans la région d’Arrabida (Portugal), 197 Mus spretus et 29 Crocidura russula ont été capturés. 11,1 % des M. spretus et 31 % des C. russula ont des trypanosomes qui n’avaient pas encore été signalés chez ces hôtes. Les différentes formes du parasite observées dans le foie d’une Crocidure trouvée morte sont décrites. L’infectivité chez des rongeurs expérimentaux, les gradients de densité de l’ADN et les profils enzymatiques montrent que les souches isolées de M. spretus sont identiques à Trypanosoma musculi isolé de Mus musculus. Les souches isolées de C. russula, bien qu’apparentées aux précédentes, sont plus proches de Trypanosoma microti. Ces données pourraient permettre d’étendre les recherches à la découverte des vecteurs et aux phénomènes de reproduction des trypanosomes.
Key words: Trypanosoma musculi / T. crocidurae / Herpetosoma / Life cycle / Infectivity
Mots clés : Trypanosoma musculi / T. crocidurae / Herpetosoma / Cycle biologique / Infection
© Masson, Paris 1993, transferred to Société Française de Parasitologie
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