Issue |
Ann. Parasitol. Hum. Comp.
Volume 64, Number 6, 1989
|
|
---|---|---|
Page(s) | 403 - 411 | |
DOI | https://doi.org/10.1051/parasite/1989646403 | |
Published online | 19 August 2016 |
Mémoire
Toxoplasma gondii: Étude ultrastructurale des formations kystiques observées en culture de fibroblastes humains
Toxoplasma gondii: Ultrastructure of cyst-like forms observed in human fibroblasts cell culture
1
Service de Parasitologie., France.
2
Service d’Anatomie pathologique I. CHU Dupuytren, F 87042 Limoges Cedex., France.
Accepté : 15 Septembre 1989
L’inoculation de cultures de fibroblastes humains par une souche de Toxoplasma gondii non pathogène pour la souris (souche Prugniaud, Limoges, 1964) donne naissance à des formations d’aspect kystique. Leur ultrastructure est comparée d’une part, à celle des kystes cérébraux chez la souris, d’autre part, pour la même souche de T. gondii, à celle des colonies intracellulaires de tachyzoïtes obtenues dans les cellules sarcomateuses TG 180 de souris.
La microscopie électronique révèle les similitudes entre les formations kystiques observées in vitro et les kystes cérébraux observés chez la souris.
Abstract
A strain of Toxoplasma gondii, non pathogenic to mice (Prugniaud strain) produced cyst-like stages when inoculated in human fibroblast culture.
An ultrastructural study was performed in order to compare these cyst-like forms to brain cysts of the same strain and to intracellular clusters of tachyzoites observed in mice TG 180 sarcoma cells.
Electron microscopy confirmed the similarities between brain cysts and cysts obtained in vitro.
Mots clés : Toxoplasma gondii / Ultrastructure / Culture cellulaire / Fibroblastes / Kystes / Cellules TG 180
Key words: Toxoplasma gondii / Ultrastructure / Cell culture / Fibroblasts / Cysts / TG 180 sarcoma cells
© Masson, Paris 1989, transferred to Société Française de Parasitologie
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