Issue |
Ann. Parasitol. Hum. Comp.
Volume 64, Number 1, 1989
|
|
---|---|---|
Page(s) | 72 - 76 | |
DOI | https://doi.org/10.1051/parasite/198964172 | |
Published online | 19 August 2016 |
Mémoire
Anopheles sergenti (Theobald) a potential malaria vector in Egypt1
Anopheles sergenti (Theobald) vecteur potentiel du paludisme en Égypte.
1
Research and Training Center on Vectors of Diseases. Ain Shams University, Abbassia Cairo, Egypt.
2
National Institutes of Health (NIH) resident consultant to Ain Shams Center.
3
Senior Scientist, Laboratory of Parasitic Diseases, NIAID, NIH.
Accepted: 11 March 1988
Two immunoassays for malaria sporozoite detection and identification, the immuno-radiometric assay (IRMA) and the enzyme-linked immunosorbent assay (ELISA) using the species-specific monoclonal antibodies are routinely performed in our laboratory. We analyzed (573) anopheline mosquitoes of A. sergenti (463), A. pharoensis (81) and A. multicolor (29) collected from Siwa-oases and Faiyum Governorate (two known active malaria foci in Egypt), for detection of P. falciparum and P. vivax sporozoites.
P. falciparum sporozoites were detected by both IRMA and ELISA tests in two A. sergenti mosquitoes (one from Siwa 1/389 = (0.26 %) and one from Faiyum Governorate 1/74 = (1.35 %)). No P. vivax sporozoites were detected. This finding is important in explaining the malaria transmission and provide first incrimination of An. sergenti as the responsible vector of malaria in Siwa-oasis, Egypt.
Résumé
La détection et l’identification de sporozoïtes responsables de la transmission du paludisme sont couramment effectuées dans notre laboratoire à l’aide de deux tests immunologiques : le test immunoradiométrique (IRMA) et l’ELISA. Nous avons ainsi testé la présence de sporozoïtes de P. falciparum et P. vivax chez 573 anophèles, dont 463 A. sergenti, 81 A. pharoensis, 29 A. multicolor. Ces moustiques ont été capturés, soit à l’Oasis de Siwa, soit à celle de Fayoum, ces deux oasis présentant un paludisme endémique.
Les tests immunologiques IRMA et ELISA ont permis de détecter des sporozoïtes de P. falciparum chez deux A. sergenti, l’un provenant de Siwa 1/389 (= 0,26 %), l’autre de Fayoum 1/74 (= 1,35 %). Des sporozoïtes de P. vivax n’ont pas été observés. Ces résultats sont importants car ils permettent d’incriminer A. sergenti à Siwa, comme responsable de la transmission du paludisme.
Key words: Anopheles sergenti / Egypt
Mots clés : Anopheles sergenti / Égypte
This study was supported by research contract entitled : « Epidemiology and Control of Arthropod-Borne Diseases in Egypt » NO1 AI 22667 NIH-NIAID between the National Institutes of Health (NIH), Bethesda Maryland, USA, and the Ain Shams Research and Training Center on Vectors of Diseases, Cairo, Egypt. The study was also supported by International Atomic Energy Agency (IAEA) project No. 3974/RB. Employment of nuclear and related techniques for monitoring « Malaria Vectors ».
© Masson, Paris 1989, transferred to Société Française de Parasitologie
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