Issue |
Ann. Parasitol. Hum. Comp.
Volume 64, Number 1, 1989
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|
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Page(s) | 3 - 9 | |
DOI | https://doi.org/10.1051/parasite/19896413 | |
Published online | 19 August 2016 |
Mémoire
Leishmania (Viannia) naiffi sp. n., a parasite of the armadillo, Dasypus novemcinctus (L.) in Amazonian brazil
Leishmania (Viannia) naiffi n. sp., parasite du tatou, Dasypus novemcinctus (L.) en Amazonie brésilienne.
The Wellcome Parasitology Unit, Caixa Postal 3, 66.001 Belém, Para, Brazil.
Accepted: 5 August 1988
A new leishmanial parasite, Leishmania (Viannia) naiffi sp. n., is described from the nine-banded armadillo, Dasypus novemcinctus (Edentata : Dasypodidae), from Para State, north Brazil. The parasite grows luxuriantly in Diffco blood-agar medium (B47), but poorly in the skin of intradermally inoculated hamsters. A comparison of isoenzyme profiles by starch gel electrophoresis separates the parasite from L. (V) braziliensis and L. (V.) guyanensis by the enzymes ASAT, ALAT, PGM, GPI, G6PD, PEP, MPI and GD, and from Leishmania (Leishmania) chagasi, L. (L.) amazonensis and L. (L.) deanei by ASAT, ALAT, PGM, GPI, MPI, G6PD, MDH, PEP and ACON. Finally, L. (V.) naiffi is serologically differentiated from L. (V.) braziliensis, L. (V.) guyanensis and L. (V.) panamensis on monoclonal antibodies specific for these parasites.
Résumé
Leishmania (Viannia) naiffi sp. n. est décrit chez un tatou, Dasypus novemcinctus (L.) provenant de l’État du Para, Brésil. Le parasite se cultive très bien sur le milieu NNN (Diffco 47), mais se développe très lentement après inoculation intradermique au Hamster. L. (V.) naiffi se sépare de L. (V.) braziliensis et L. (V.) guyanensis par les profils enzymatiques des enzymes : ASAT, ALAT, PGM, GPI, G6PD, PEP, MPI et GD. Il se sépare de L. (Leishmania) chagasi, L. (L.) amazonensis et L. (L.) deanei par les profils enzymatiques de ASAT, ALAT, PGM, GPI, MPI, G6PD, MDH, PEP et ACON. Enfin, le nouveau parasite se distingue de L. (V.) braziliensis, L. (V.) guyanensis et L. (V.) panamensis grâce aux anticorps monoclonaux.
Key words: Leishmania naiffi sp. n. / Armadillo / Brazilian amazonas
Mots clés : Leishmania naiffi. n. sp. / Tatou / Amazonie brésilienne
© Masson, Paris 1989, transferred to Société Française de Parasitologie
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