| Issue |
Ann. Parasitol. Hum. Comp.
Volume 59, Number 5, 1984
|
|
|---|---|---|
| Page(s) | 525 - 526 | |
| DOI | https://doi.org/10.1051/parasite/1984595525 | |
| Published online | 12 September 2017 | |
Note préliminaire
Obtention in vitro des stades hépatiques de Plasmodium falciparum
In vitro cultivation of the exoerythrocytic stages of Plasmodium falciparum.
1
Département de Parasitologie et de Médecine tropicale, Groupe Hospitalier Pitié-Salpêtrière, 47 Boulevard de l'Hôpital, F 75651 Paris Cedex 13, France.
2
NM RI, Bethesda, Maryland.
3
Laboratoire de Zoologie-Vers, associé au CNRS, Muséum National d'Histoire Naturelle, 61 rue Buffon, F 75231 Paris Cedex 05, France.
4
Unité de Recherches Hépatologiques-U 49, INSERM, Hôpital Pontchaillou, F 35011 Rennes, France.
5
Service de Chirurgie, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
Accepté : 31 Juillet 1984
Abstract
Sporozoites of Plasmodium falciparum, obtained by membrane feeding of Anopheles freeborni or A. stephensi with cultured gametocytes, were used to infect monolayers of human hépatocytes. Fluorescent labelling with an African serum as well as Giemsa staining performed from day one to day 7 of cultures, demonstrated the presence of numerous hepatic schizonts measuring up to 40 µm.
© Masson, Paris 1984, transferred to Société Française de Parasitologie
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