Note préliminaire

Infestation in vitro d’hepatocytes humains par des sporozoites de Plasmodium vivax : schizogonie et libération de mérozoïtes infestants pour des hématies humaines

In vitro infestation of human hepatocytes by Plasmodium vivax sporozoïtes ; maturation of schizonts and release of mérozoïtes which invaded human erythrocytes

¹ Département de Parasitologie et de Médecine tropicale, Groupe Hospitalier Pitié-Salpêtrière, 47 boulevard de l’Hôpital, F 75651 Paris Cedex 13, France.
² Laboratoire de Zoologie-Vers, associé au CNRS, Muséum National d’Histoire Naturelle, 61, rue Buffon, F 75231 Paris Cedex 05, France.
³ Unité de Recherches Hépatologiques - U 49, INSERM, Hôpital Pontchaillou, F 35011 Rennes, France.
⁴ Service de Chirurgie, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.

Accepté : 27 Juin 1983

Abstract

Human hepatocytes obtained by perfusion of a liver biopsy fragment, were infected in vitro with Plasmodium vivax sporozoites raised in Anopheles stephensi. On day 7 of culture, numerous hepatocytic schizonts ranging in size from 5 to 35 ìm were observed. The schizont maturation was complete and led to the release of invasive merozoites as shown by in vitro infestation of human red cells layered on the hepatocytes cultures.