Issue |
Ann. Parasitol. Hum. Comp.
Volume 57, Number 6, 1982
|
|
---|---|---|
Page(s) | 527 - 531 | |
DOI | https://doi.org/10.1051/parasite/1982576527 | |
Published online | 15 September 2017 |
Mémoire
Typage enzymatique de onze souches de Leishmania isolées, en Italie continentale, à partir de formes viscérales murines, canines et vulpines
Mise en évidence d’un variant enzymatique chez le Renard (Vulpes vulpes) et le Chien
Enzymatic typing of 11 isolates of Leishmania collected from visceral infections in rodents, dogs and foxes on the Italian mainland
Demonstration of an enzyme variant in the parasite of the fox (Vulpes vulpes) and dog
1 Laboratorio di Parassitologia, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Roma, Italie
2 Laboratoire d’Écologie médicale (Pr. J. A. Rioux), Faculté de Médecine, 163, rue Auguste-Broussonnet, F 34000 Montpellier, France
3 Department of Medicai Protozoology, London School of Hygiene and Tropical Medicine (Pr. W. Peters), Keppel Street (Gower Street), Londres WC1E 7HT, U.K.
4 Cattedra di Parassitologia (Pr. S. Bettini), Facoltà di Scienze, Università di Cagliari, 09100 Cagliari, Italie
Accepté : 8 Juillet 1982
Le typage enzymatique de 11 souches de Leishmanies, isolées en Italie (Toscane), chez le Chien (6 souches), le Renard (Vulpes vulpes, 1 souche) et le Rat noir (Rattus rattus, 4 souches) a été effectué indépendemment à Montpellier et à Londres, au moyen des techniques d’électrophorèse en gel d’amidon. Ont été testées : à Montpellier, ME, G-6-PD, 6-PGD, PGM, PGI, GOT (= ASAT), MDH et IDH ; à Londres, ME, 6-PGD, PGM, PGI, ASAT, ALAT, SOD, NH et MPI. Le résultat est identique pour les deux laboratoires : la totalité des souches isolées du Rat et 4 souches isolées du Chien sont rapportées à Leishmania infantum s. st. (zymodème 1 ). En revanche, trois souches, dont deux isolées du Chien et une du Renard, correspondent à un variant enzymatique (zymodème 18).
Ces résultats attirent une nouvelle fois l’attention sur la diversité taxonomique des parasites responsables de la leishmaniose viscérale.
Abstract
Enzyme typing by starch gel electrophoretic techniques was carried out on 11 Leishmania isolates from Italy in laboratories in Montpellier and London. The enzymes studied in the former were ME, G-6-PD, 6-PGD, PGM, PGI, GOT (= ASAT), MDH and IDH. In London slightly different procedures were used to examine ME, 6-PGD, PGM, PGI and ASAT. In addition, SOD, ALAT, NH, and MPI were studied. The combined data revealed that four Rattus raltus and four dogs were infected with classical L. infantum (zymodème 1). In addition, two other dogs and a fox were infected with an enzyme variant (zymodème 18) which differed in four of the 12 enzymes examined. These findings raise the question of the heterogeneity of the parasites causing visceral leishmaniasis.
© Masson, Paris 1982, transferred to Société Française de Parasitologie
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