| Issue |
Ann. Parasitol. Hum. Comp.
Volume 56, Number 5, 1981
|
|
|---|---|---|
| Page(s) | 467 - 475 | |
| DOI | https://doi.org/10.1051/parasite/1981565467 | |
| Published online | 21 September 2017 | |
Mémoire
Signification du polymorphisme enzymatique chez les leishmanies
A propos de trois souches hétérozygotes de Leishmania infantum Nicolle, 1908, Leishmania cf. tarentolae Wenyon, 1921 et Leishmania æthiopica Bray, Ashford et Bray, 1973
Significance of enzymatic polymorphism in Leishmaniae
On three heterozygous stocks of Leishmania infantum Nicolle, 1908, Leishmania cf. tarentolae Wenyon, 1921 and Leishmania æthiopica Bray, Ashford and Bray, 1973
1 Laboratoire d’Écologie médicale et Pathologie parasitaire (Pr. J. A. Rioux), Faculté de Médecine, rue Auguste Broussonnet, F 34000 Montpellier, France
2 Medical Research Council (External Staff), Department of Pure and Applied Biology, Imperial College, London Sw 7 2 AZ, England
Accepté : 17 Février 1981
L’analyse isoenzymatique de 49 souches de Leishmanies [35 Leishmania infantum Nicolle, 1908, 10 Leishmania cf. tarentolae Wenyon, 1921, 2 Leishmania tropica (Wright, 1903) et 2 Leishmania æthiopica Bray, Ashford et Bray, 1973] a révélé l’existence de structures hétérozygotes pour trois d’entre elles. Sur huit systèmes enzymatiques éprouvés : (PGM, PGI, G-6-PDH, 6-PGDH, IDH, MDH, ME et GOT), trois d’entre eux se sont exprimés par deux (PGM) ou trois (PGI et 6-PGDH) taches sur les gels d’électrophorèse. Une souche de L. infantum (Chien, Cévennes) et une souche de L. cf. tarentolae [Sergentomyia minuta (Rondani, 1843)] étaient hétérozygotes pour la PGI. Une souche de L. æthiopica (Homme, Ethiopie) était hétérozygote pour la PGI, la PGM et la 6-PGDH. Les images observées, comparables à celles obtenues avec les organismes eucaryotes à reproduction sexuée, permettent de suspecter, chez les Leishmanies, l’existence de structures diploïdes et de processus d’échanges génétiques.
Abstract
The analysis of 8 isoenzymes (PGM, PGI, G-6-PDH, 6-PGDH, IDH, MDH, ME, GOT) of 49 stocks of leishmaniae [35 of Leishmania infantum Nicolle, 1908,10 of Leishmania cf. tarentolae Wenyon, 1921, 2 of Leishmania tropica (Wright, 1903) and 2 of Leishmania æthiopica Bray, Ashford and Bray, 1973] revealed heterozygous structures in three stocks. These structures were expressed by two (PGM) or three (PGI, 6-PGDH) spots on the electrophoretic gels. Two stocks were heterozygous for PGI alone [one of L. infantum from a dog in the Cévennes, France; one of presumed Leishmania tarentolae from Sergentomyia minuta (Rondani, 1843) from P. O., France] and one was heterozygous for PGI, PGM and 6-PGDH (L. æthiopica from man in Ethiopia). The results are comparable to those seen in eukaryotic organism with sexual reproduction and suggest that leishmaniae have diploid structures and a process of genetic exchange.
© Masson, Paris 1981, transferred to Société Française de Parasitologie
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