Open Access
Short Note
Issue
Parasite
Volume 24, 2017
Article Number 10
Number of page(s) 4
DOI https://doi.org/10.1051/parasite/2017010
Published online 21 March 2017

© S. Wang et al., published by EDP Sciences, 2017

Licence Creative Commons
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction

Toxoplasma gondii is an obligate intracellular protozoan parasite that has a worldwide distribution and infects a wide range of warm-blooded vertebrates, including humans [3]. The ingestion of undercooked meat containing tissue cysts and the ingestion of food or drink contaminated with sporulated T. gondii oocysts are important modes of transmission of T. gondii. As definitive hosts of this parasite, cats play an important role in the life cycle of T. gondii, facilitating the genetic recombination between strains, as well as environmental contamination [12]. Antibodies to T. gondii have been reported in cats worldwide and seroprevalence in cats was recently summarized [2, 4, 8, 9]. The objective of the present survey was to determine the seroprevalence of T. gondii in domestic cats in Henan province, central China, and to evaluate the main associated risk factors related to exposure to T. gondii in this region [6, 14].

Materials and methods

Ethics statement

The study was reviewed and approved by the Ethics Review Committee of the Xinxiang Medical University (Reference No. 2015016).

Study site

The study was conducted in Henan province in the central part of mainland China, covering an area of 167, 000 km2 and having a population of approximately 106 million. Its geographical position is at east longitude 110°21′–116°39′ and at north latitude 31°23′–36°22′. The Yellow River passes through central Henan. The area has a continental monsoon climate, with four distinctive seasons. The average annual temperature is 12.1–15.7 °C, with a mean annual rainfall of 532.5–1380.6 mm. There are 17 provincial cities distributed in Henan province, with the city of Zhengzhou as its capital. Five cities, including Anyang (35°13′–36°22′ N, 113°37′–114°58′ E), Sanmenxia (33°31′–35°05′ N, 110°21′–112°01′ E), Zhengzhou (34°16′–34°58′ N, 112°42′–114°13′ E), Xinyang (31°46′–31°52′ N, 114°01′–114°06′ E), and Shangqiu (33°43′–34°52′ N, 114°49′–116°39′ E), located in the northern, western, central, southern, and eastern parts of Henan province, were selected for sample collections.

Sample collection

A total of 843 blood samples of domestic cats were collected from these five cities in Henan province between March 2015 and May 2016. Cat owners were asked for details of the animal’s age, sex, source, and breed using a structured questionnaire. Blood samples were centrifuged and sera were recovered and transferred to 1.5 mL Eppendorf tubes. All the sera were then stored at −80 °C until tested for anti-T. gondii antibodies.

Determination of antibodies to T. gondii

Antibodies to T. gondii were determined using the commercial T. gondii IgG ELISA (enzyme-linked immunosorbent assay) Kit (Combined Company, Shenzhen, Guangdong Province, China) according to the manufacturer’s instructions [5, 13]. The manufacturer’s test report showed that the sensitivity and specificity of this ELISA kit were 95% and 100%, respectively. Positive and negative control sera were provided in the kit. Briefly, the T. gondii specific antigen was used to coat a 96-well ELISA plate. After incubation of the diluted serum sample (1:100) in the test well and subsequent washing, a conjugate was added. The plate was washed again and the chromogenic enzyme substrate was added. Finally, the optical density (OD) values of each well were measured using an ELISA plate reader (Multiskan MK3; Thermo Scientific, Waltham, MA, USA) at 450 nm.

A relative rate percent (IRPC) value was obtained using the following formula:

The sera were considered negative to T. gondii if IRPC < 2.1 and positive if IRPC > 2.1.

Statistical analysis

Differences in T. gondii prevalence for different variables such as age, breed, and sex were analyzed using a chi square test. Statistical analysis was performed using SPSS 20 software for Windows (SPSS Inc., Chicago, Illinois, USA). The differences were considered statistically significant if p < 0.05.

Results

Antibodies to T. gondii were detected in 21.12% (178/843) of cats (Table 1). Seropositivity percentages from different cities were: 21.84% of 174 from Anyang, 14.56% of 158 from Sanmenxia, 30.54% of 167 from Zhengzhou, 17.68% of 181 from Xinyang, and 20.86% of 163 from Shangqiu.

Table 1.

Seroprevalence of Toxoplasma gondii infection in domestic cats in Henan province, central China.

The seroprevalence of T. gondii was higher in males (22.16%, 92/416) than in females (20.14%, 86/427) but the difference was not significant (p > 0.05). The seroprevalence of T. gondii infection was 16.67% (54/324) in purebred cats and 23.89% (124/519) in mixed-breed cats, showing a significant difference by breed (p < 0.05). Moreover, significantly higher seroprevalence was found in rural cats (29.26%), compared to that of cats raised in urban area (16.35%) (p < 0.01).

The prevalence of T. gondii infection in cats increased significantly (p < 0.01) with the increase in age.

Discussion

The present survey showed that T. gondii seroprevalence in cats in rural areas was significantly higher compared to urban cats (p < 0.01), which is consistent with reports by other authors [1, 10]. The difference in seroprevalence in urban and rural areas might be explained by differences in local reservoirs of the parasite, both in prey animals and in the environment, which serve as local infection sources for the cats. In Henan, domestic cats are usually kept outdoors in rural areas and often roam more freely with greater access to parasites. In addition, the number of rodents in rural areas is much higher than that in urban areas. Higher T. gondii seroprevalence found in cats in rural areas probably results from the carnivorous behavior of cats living outdoors and eating prey animals such as rodents and birds. The higher seropositivity with increasing age of the cats supports the hypothesis that most cats acquire T. gondii infection after weaning [1, 7, 13].

In order to protect public health, more measures should be taken. The proper disposal of cat litter, keeping cats indoors to minimize their acquisition of infection from prey or the environment, and reducing the feral cat population are recommended. Reducing close contact with cats and protecting the play areas of children might potentially reduce the oocyst burden [11]. What is more, children, pregnant women, and immunocompromised people should adhere to hygiene principles after contact with soil and cats. Given the high seroprevalence of T. gondii IgG antibodies in cats, performing a screening test and determination of the IgG antibody titer in high-risk populations such as pregnant women in Henan province, central China is recommended.

Conflict of interest

The authors declare that they have no conflict of interest.

Acknowledgments

This study was supported by the Doctoral Scientific Research Activation Foundation of Xinxiang Medical University (Nos. XYBSKYZZ201504, XYBSKYZZ201631, and XYBSKYZZ201603), the support project for the Disciplinary group of Psychology and Neuroscience, Xinxiang Medical University (2016PN-KFKT-26), and the Medical Science and Technology Research Project of Henan Province (No. 200903081).

References

  1. Ahmad N, Ahmed H, Irum S, Qayyum M. 2014. Seroprevalence of IgG and IgM antibodies and associated risk factors for toxoplasmosis in cats and dogs from sub-tropical arid parts of Pakistan. Tropical Biomedicine, 31(4), 777–784. [PubMed] (In the text)
  2. Alvarado-Esquivel C, Romero-Salas D, Cruz-Romero A, Garcia-Vazquez Z, Peniche-Cardena A, Ibarra-Priego N, Ahuja-Aguirre C, Perez-de-Leon AA, Dubey JP. 2014. High prevalence of Toxoplasma gondii antibodies in dogs in Veracruz, Mexico. BMC Veterinary Research, 10, 191. [CrossRef] [PubMed] (In the text)
  3. Dubey JP, Jones JL. 2008. Toxoplasma gondii infection in humans and animals in the United States. International Journal for Parasitology, 38(11), 1257–1278. [CrossRef] [PubMed] (In the text)
  4. Lopes AP, Santos H, Neto F, Rodrigues M, Kwok OC, Dubey JP, Cardoso L. 2011. Prevalence of antibodies to Toxoplasma gondii in dogs from northeastern Portugal. Journal of Parasitology, 97(3), 418–420. [CrossRef] (In the text)
  5. Liu QX, Wang S, Wang LQ, Xing J, Gao WJ, Liu GF, Zhao B, Zhang HB, Gao LH. 2014. Seroprevalence of Toxoplasma gondii infection in dogs and cats in Zhenjiang City, Eastern China. Asian Pacific Journal of Tropical Biomedicine, 4(9), 725–728. [CrossRef] (In the text)
  6. Li F, Wang S-P, Wang C-J, He S-C, Wu X, Liu G-H. 2016. Seroprevalence of Toxoplasma gondii in goats in Hunan province, China. Parasite, 23, 44. [CrossRef] [EDP Sciences] [PubMed] (In the text)
  7. Must K, Lassen B, Jokelainen P. 2015. Seroprevalence of and risk factors for Toxoplasma gondii infection in cats in Estonia. Vector-Borne and Zoonotic Diseases, 15(10), 597–601. [CrossRef] (In the text)
  8. Machacova T, Bartova E, Sedlak K, Slezakova R, Budikova M, Piantedosi D, Veneziano V. 2016. Seroprevalence and risk factors of infections with Neospora caninum and Toxoplasma gondii in hunting dogs from Campania region, southern Italy. Folia Parasitologica, 63, 12. [CrossRef] (In the text)
  9. Nguyen TT, Choe SE, Byun JW, Koh HB, Lee HS, Kang SW. 2012. Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea. Acta Parasitologica, 57(1), 7–12. [CrossRef] [PubMed] (In the text)
  10. Oi M, Yoshikawa S, Maruyama S, Nogami S. 2015. Comparison of Toxoplasma gondii seroprevalence in shelter cats and dogs during 1999–2001 and 2009–2011 in Tokyo, Japan. PLoS One, 10(8), e0135956. [CrossRef] (In the text)
  11. Torrey EF, Yolken RH. 2013. Toxoplasma oocysts as a public health problem. Trends in Parasitology, 29(8), 380–384. [CrossRef] [PubMed] (In the text)
  12. Vilares A, Gargate MJ, Ferreira I, Martins S, Julio C, Waap H, Angelo H, Gomes JP. 2014. Isolation and molecular characterization of Toxoplasma gondii isolated from pigeons and stray cats in Lisbon, Portugal. Veterinary Parasitology, 205(3–4), 506–511. [CrossRef] [PubMed] (In the text)
  13. Zhang H, Zhou DH, Zhou P, Lun ZR, Chen XG, Lin RQ, Yuan ZG, Zhu XQ. 2009. Seroprevalence of Toxoplasma gondii infection in stray and household cats in Guangzhou, China. Zoonoses and Public Health, 56(9–10), 502–505. [CrossRef] [PubMed] (In the text)
  14. Zhang N, Wang S, Wang D, Li C, Zhang Z, Yao Z, Li T, Xie Q, Liu S, Zhang H. 2016. Seroprevalence of Toxoplasma gondii infection and risk factors in domestic sheep in Henan province, central China. Parasite, 23, 53. [CrossRef] [EDP Sciences] [PubMed] (In the text)

Cite this article as: Wang S, Zhou Y, Niu J, Xie Q, Xiao T, Chen Y, Li H, Ma C, Zhang H, Liu S & Zhang Z: Seroprevalence of Toxoplasma gondii infection in domestic cats in central China. Parasite, 2017, 24, 10.

All Tables

Table 1.

Seroprevalence of Toxoplasma gondii infection in domestic cats in Henan province, central China.

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