Seroprevalence of Neospora caninum in dairy cattle from north-west and centre of Romania

Neosporosis is a disease that mainly affects cattle in both dairy and beef herds. The main definitive host of this parasite is the dog. Since 1984 and its first description a large number of data were published worldwide on this parasite. In Romania, the research regarding this parasite is limited. The purpose of this study was to determine the seroprevalence of Neospora caninum infection in dairy cattle from six regions in north-western Romania and to evaluate the intensity of infection in different animals groups. A total number of 901 samples (862 sera from adult cows and 39 sera from calves) were collected from dairy farms and were screened for the presence of specific IgG anti-bodies using an enzyme-linked immunosorbent assay (ELISA). The overall seroprevalence for neosporosis was 34.6%. In adult cows and calves seroprevalences reached 34.8% (300/862) and 30.8% for calves (12/39) respectively. In cattle which had previously aborted, seroprevalence was 40.9%. These results indicate that N. caninum infection is widespread among animals reared in dairy systems from Romania and a program for farmer training and a strategy for reducing the economic impact of the disease are needed.

Neosporosis is a disease that mainly affects cattle in both dairy and beef herds. The main definitive host of this parasite is the dog.
Since 1984 and its first description a large number of data were published worldwide on this parasite. In Romania, the research regarding this parasite is limited. The purpose of this study was to determine the seroprevalence of Neospora caninum infection in dairy cattle from six regions in north-western Romania and to evaluate the intensity of infection in different animals groups. A total number of 901 samples (862 sera from adult cows and 39 sera from calves) were collected from dairy farms and were screened for the presence of specific IgG anti-bodies using an enzymelinked immunosorbent assay (ELISA As intermediate hosts, it affects mainly bovines causing abortion and birth of chronically infected calves that implies important economic losses mainly in dairy industry. The abortion can take place from the third month until the last month of pregnancy but mostly occurs at five-six months of gestation (Moore, 2005). The economical losses are determined by the pregnancy loss, lower production of milk, rebreeding of the animal and medical assistance and costs determined by the eventual replace of the infected animal (Dubey et al., 2007). Other intermediate hosts can be represented by ovines, goats, dears, buffalos, and many others animals. As definitive hosts dogs, coyotes and Australian dingoes have been identified (McAllister et al., 1998;Gondim et al., 2004;King et al., 2010). Worldwide the seroprevalence of neosporosis in cattle varies and different values were obtained: 5.7 % in Japan (Koiwai et al., 2006), 13.9 % in Uruguay (Banales et al., 2006), 11.3 % in Venezuela (Lista-Alves et al., 2006), 20.1 % in Slovakia (Reiterova et al., 2009), 24.1 % in Spain (Panadero et al., 2010 and 2.8 % in Sweden (Loobuyck et al., 2009). In Romania the first report regarding Neospora caninum infection in cattle was in 2002(Ionescu et al., 2002 showing a prevalence of 20 % (30/150 animals). The next year a larger study was in conducted and bovine sera were tested giving a seroprevalence of 33.71 % (Ionescu et al., 2003). In this study a larger number of samples were taken under study and they were collected from a larger Research note Parasite, 2011, 18, 349-351 number of counties providing a much more reliable result regarding the spread of neosporosis in cattle from Romania.

MATERIALS AND METHODS
B etween 2008 and 2010, a total number of 901 sera samples were collected from 16 dairy farms. These farms were located in six different counties from central and northwestern parts of Romania: Alba, Cluj, Maramureş, Mureş, Sibiu and Satu Mare. The lowest number of samples collected from a farm was six and the highest was 188. All the sampled farms had vaccination schemes against brucellosis, leptospirosis, infectious bovine rhinotracheitis and bovine viral diarrhea. The blood was collected by puncture from jugular or caudal vein and, incubated at 37 °C and then centrifuged for serum collection. Sera were kept under -20 °C until further use. All sera were tested for antibodies against N. caninum by using a commercial enzyme immuno-assay kit Neospora caninum Antibody Test Kit produced by IDEXX Laboratories, Switzerland. Briefly, each serum sample (diluted 1:100) was added to the Neospora antigen-coated microplates and incubated for 30 minutes at room temperature. Then, the antigen provided in the kit was added in each well and incubated at room temperature for   another 30 minutes. The reaction was revealed by adding the chromogen and incubating for 15 minutes at room temperature. The color reaction was stopped by adding stop solution. Plates were read at 650 nm and the results were expressed as optical density (OD) values and were analyzed using the formulas provided by the manufacturer. Using a statistical package (EpiInfo Version 5) the p-value was also calculated.

RESULTS
A ntibodies against N. caninum were detected in 312 (34.6 %) serum samples from 901 pure breed cows. No results were considered as being doubtful. The average within-heard seroprevalence was 31.11 %, with a range between 11.1 and 60.0 % in seropositive farms (Table I). In adult cows and calves the seroprevalence were 34.8 % (300/862) and 30.8 % (12/39) respectively.
Out of the 901 animals tested, 137 (15,2 %) had at least one abortion in their history. The seroprevalence of N. caninum infection in these animals reached a value of 40.9 % (95 % CI 32.6-49.6). Amongst the rest of the animals that had no previous reproduction problems the seroprevalence was 33.5 % (256/764). The difference between these two animal categories was statistically significant: p < 0.05 (Table II). Also the tested animals were divided into two other categories: animals, which had no pregnancy, and animals that had at least one pregnancy in their history. From the first group, (176 animals), 77 presented antibodies against N. caninum, reaching a percentage of 43.8. From the second group (725 animals), 32.4 % were positive. These results were also statistically significant: p < 0.004.  (Romero et al., 2005) and Mexico (42 %) (Garcia-Vazquez et al., 2005). In the present herd seroprevalences varied between 11.1 and 60.0 % and the differences were statistically significant between herds. The fact that all the farms taken under study had cattle positive for N. caninum infection indicates that this infection in dairy cattle is widespread in Romania. There have been many studies that analysed and demonstrated the association between seroprevalence of neosporosis and abortion. Although the association between abortion and N. caninum seropositivity was not strong (odds ratio, 0.72) there was some evidence of neosporosis-associated abortions. A relatively high seroprevalence of N. caninum was recorded in animals that aborted. There was also a significant difference between animals that had no recorded pregnancies and the ones that had at least one pregnancy in their history.