Study design diagram. 12 dogs were allocated to either an untreated control group or a treated group receiving Vectra 3D^®. Each group was further divided into two sub-groups (A and B) to allow staggered experimental procedures. Treatment was performed on Days 0 and 2 for sub-groups A and B, respectively. Dogs were combed for hair, which was then used for assessments performed on Days 1, 7, 14, 21, and 28 post-treatment for sub-group A and on Days 3, 9, 16, 23 and 30 post-treatment for sub-group B.