Open Access
Table 2
Detection of A. phagocytophilum DNA and RNA in S. calcitrans after an interrupted meal of infected blood.
| DNA detection | RNA detection# | |||
|---|---|---|---|---|
| Experiments | Mean* Ct ± SEM | Positive S. calcitrans for Aph msp2 | Mean* Ct ± SEM | Positive S. calcitrans for Aph msp2 |
| IntM1 | 25.46 ± 2.20 | 10/10 | 20.83 ± 0.38 | 9/10 |
| IntM2 | 29.63 ± 2.06 | 10/10 | 18.50 ± 0.64 | 3/10 |
| IntM3 | 28.72 ± 2.24 | 9/10 | 17.28 ± 0.27 | 3/10 |
| IntM4 | 19.04 ± 0.30 | 3/10 | N.D. | N.D. |
| IntM5 | 26.22 ± 3.96 | 5/10 | N.D. | N.D. |
| IntM6 | 28.94 ± 2.38 | 10/10 | N.D. | N.D. |
*Mean Ct on Aph-positive S. calcitrans.
#With pre-amplification.
N.D. not determined.
Aph: A. phagocytophilum.
IntM: Interrupted blood meal.
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