Processing and mounting phlebotomine sand flies: a consensus guideline

Open Access

Review

Table 5

Average cost, application, and protocol adaptation for the extraction of gDNA of phlebotomine sand flies.

Protocol	Cost	Application	Protocol adaptation for small arthropods
Spin column	2.5–3.55 US$ [39]	PCR, NGS	[9]
Phenol-chloroform	0.24 US$ [69]	PCR, NGS	[9]
HotSHOT	<0.01 US$ [69]	PCR	–
Salting out	0.12 $ [69]	PCR	–
Chelex	0.02 $4 [41]	PCR	[41, 76]

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