Editing cascades of COIII mRNA, predicted from the sequencing reads of each Trypanosoma lainsoni isolate: A) Le29, B) Ca47, and C) Ca37. Guide RNAs are shown aligned below the COIII mRNA (light blue line) according to their editing position. The x-axis represents the mRNA position, while the y-axis shows the cumulative number of gRNA classes up to that position. gRNA classes are color-coded according to sequence abundance as follows: red (1–19 reads), black to green (20–1,000 reads), and light green (>1,000 reads).