Schematic representation of the Trypanosoma lainsoni minicircle (1,184 bp) from Le29 isolate. The outermost ring (yellow) highlights the positions of the conserved sequence blocks (CSB-1, CSB-2, and CSB-3) in each CR (green segments). The inner ring (grey) shows the expected PCR amplification product obtained with the primers LaimHVR_L and LaimHVR_R (red), which bind to the conserved regions flanking the mHVRs (blue segments).