Conserved Region alignments. A) Sequence logo of Trypanosoma lainsoni kDNA minicircle conserved region. Nucleotide frequency in each position of a multiple alignment of minicircle assembled sequences is shown. Each base is represented by its corresponding letter and the height of each symbol represents its frequency. Dashed lines indicate CSB-1, CSB-2, and CSB-3 conserved blocks. B) Sequence comparison of conserved blocks shared by different trypanosomatids species. CSB-1, CSB-2, and CSB-3 blocks in T. lainsoni minicircles are highly conserved against the trypanosomatids conserved blocks consensus sequence. However, CSB-1 presents a substitution at the first position (A → G). The CSB-1/CSB-2 distance is conserved in T. lainsoni with respect to T. brucei, T. lewisi, L. braziliensis, and L. orientalis, while the CSB-2/CSB-3 distance is variable, being similar to Leishmania and two nucleotides shorter than in T. cruzi.