Length frequency histograms of Trypanosoma lainsoni minicircles. A) KOMICS-circularized contigs were grouped into two categories based on their length (in base pairs): short contigs (blue shades, 25%) and long contigs (purple shades, 75%). Dark bars represent Le29 isolate, intermediate shades represent Ca37, and light shades represent Ca47. B) ONT reads filtered by BLAST alignment to the minicircle CR. The most frequent read lengths (600–1,600 bp) are shown. Reads between 900–1,300 bp, representing 77.3% of filtered reads, are highlighted in purple. C) Length frequency histograms of ONT-derived minicircles grouped by the number of CRs. The upper panel shows minicircles with a single CR; 79.0% of these falls within the 900–1,300 bp range (highlighted in purple). The lower panel shows minicircles with two CRs; 74.8% fall within the same range.