Open Access
Table 1
Primers and PCR conditions used in this study to barcode two parasite groups (Acanthocephala, Microsporidia) and their isopod host A. aquaticus.
| Primers (5′–…–3′) | Target | Primer reference | PCR program |
|---|---|---|---|
| V1F | Classical Microsporidia; partial 18S SSU rRNA gene | Zhu et al. [72]; Weigand et al. [67] | 94 °C, 3 min;40× (94 °C, 35 s; 68 °C, 40 s); 68 °C, 5 min |
| CACCAGGTTGATT | |||
| CTGCCTGAC | |||
| micuni3R | |||
| ATTACCGCGGMTG | |||
| CTGGCAC | |||
| LCO1490 | Metazoan invertebrates; Partial CO1 gene | Folmer et al. [17] | 94 °C, 3 min; 40× (94 °C, 40 s;58 °C, 40 s;65 °C, 50 s);30× (94 °C, 40 s;53 °C, 30 s;65 °C, 50 s);72 °C, 5 min |
| GGTCAACAAATCA | |||
| TAAAGATATTGG | |||
| HCO2198 | |||
| TAAACTTCAGGGTG | |||
| ACCAAAAAATCA |
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