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Matrix representation of a replicate of the semi-field experiment implemented at CIRAD La Bretagne Research Station (Sainte-Clotilde, La Reunion), from November 2020 to April 2021, to assess the effect of different settings of the sterile insect technique (SIT) and boosted SIT in suppressing controlled populations of Aedes albopictus. The demographic analysis included data from four replicates, with eight mosquito-netted cages by replicate. A fifth replicate was run with a spuriously high amount of pyriproxyfen to boost the sterile males, as a pyriproxyfen positive control. It was not used for the demographic analysis. Fixed proportions, coded x:y:z, of adult sterile males (x), and fertile males (y) and females (z) (“F” standing for ‘Fertile” below the code) were released in each cage. Eggs were collected in oviposition traps, counted, dried, and flooded for hatch. Immature stages were monitored until the emergence of adults, or up to 15 days after hatch. Cages A, B, E, and F (upper row of the matrix) were used to mimic the dynamics of immature populations of Aedes albopictus under two factors of standard or boosted SIT: (i) sterile-to-fertile male ratio (noted s2f) of 1:1 (cages A and E), or 5:1 (cages B and F), and (ii) exposure to pyriproxyfen vectored by the sterile males: (cages A and B), or no exposure (cages E and F). The other cages (lower row of the matrix) were used for quality control - hatching of eggs collected from control females; residual fertility in sterile males (cage G), and boosted sterile males (cage C) - capacity of sterile males to vector pyriproxyfen to the breeding sites, with respect to the presence of females (cages C and D).

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