Table 5
Proteins identified by LC-MS/MS for spots 21–24 classified according to their molecular weight and assigned to the IgM triplet band with the closest molecular weight. Proteins with too low molecular weight (below 65 kDa) were not considered to belong to the IgM triplet. High molecular weight proteins (over 100 kDa) were integrated into the IgM triplet, as protein resolution is lower in this zone (ND: not detected). Lines in bold refer to the thirteen main candidates for the IgM triplet.
| Protein name for spot No. 21 | Protein name for spot No. 22 | Protein name for spot No. 23 | Protein name for spot No. 24 | |
|---|---|---|---|---|
| Low molecular weight | SAG-related sequence SRS29B (SRS29B) | SAG-related sequence SRS29B (SRS29B) | SAG-related sequence SRS29B (SRS29B) | SAG-related sequence SRS29B (SRS29B) |
| Actin 1 (ACT1) | Actin 1 (ACT1) | Actin 1 (ACT1) | Actin 1 (ACT1) | |
| ND | Protease Inhibitor 1 (PI1) | ND | ND | |
| Microneme protein 1 (MIC1) | ND | Microneme protein 1 (MIC1) | ND | |
| Alpha Tubulin A1 (TUBA1) | ND | ND | ND | |
| ND | Alveolin domain containing intermediate filament IMC4 (ALV4) | ND | ND | |
| Protein disulfide isomerase | ND | Protein disulfide isomerase | Protein disulfide isomerase | |
| Histone lysine methyltransferase, SET, putative | Histone lysine methyltransferase, SET, putative | ND | ND | |
| ND |
ND |
Alveolin domain containing intermediate filament IMC3 (ALV3) |
ND |
|
| 75 kDa band | EMP24/GP25L/p24 family protein | EMP24/GP25L/p24 family protein | EMP24/GP25L/p24 family protein | EMP24/GP25L/p24 family protein |
| ND | ND | ND | D-3-phosphoglycerate dehydrogenase | |
| ND | ND | ATPase, AAA family protein | ATPase, AAA family protein | |
| Vacuolar ATP synthase subunit A, putative | Vacuolar ATP synthase subunit A, putative | Vacuolar ATP synthase subunit A, putative | Vacuolar ATP synthase subunit A, putative | |
| Alveolin domain containing intermediate filament IMC1 (ALV1) | Alveolin domain containing intermediate filament IMC1 (ALV1) | Alveolin domain containing intermediate filament IMC1 (ALV1) | Alveolin domain containing intermediate filament IMC1 (ALV1) | |
| ND | ND | Chaperonin cpn60, putative | Chaperonin cpn60, putative | |
| Heat Shock Protein 70 (HSP70) | Heat Shock Protein 70 (HSP70) | Heat Shock Protein 70 (HSP70) | Heat Shock Protein 70 (HSP70) | |
| ND | ND | ND | RNA recognition motif-containing protein | |
| Chaperonin protein BiP | Chaperonin protein BiP | Chaperonin protein BiP | Chaperonin protein BiP | |
| Hypothetical protein | Hypothetical protein | Hypothetical protein | Hypothetical protein | |
| Tryptophanyl-tRNA synthetase (TrpRS2) | Tryptophanyl-tRNA synthetase (TrpRS2) | Tryptophanyl-tRNA synthetase (TrpRS2) | Tryptophanyl-tRNA synthetase (TrpRS2) | |
|
Heat Shock Protein (HSP)
|
Heat Shock Protein (HSP)
|
Heat Shock Protein (HSP)
|
Heat Shock Protein (HSP)
|
|
| 90 KDa band | Heat Shock Protein 90 (HSP90) | Heat Shock Protein 90 (HSP90) | Heat Shock Protein 90 (HSP90) | Heat Shock Protein 90 (HSP90) |
| Hypothetical protein | Hypothetical protein | Hypothetical protein | ND | |
| Tetratricopeptide repeat-containing protein | ND | ND | ND | |
| ND | ND | Cell Division protein C48CY (CDC48CY) | Cell Division protein C48CY (CDC48CY) | |
| ND | ND | Dynamin-Related Protein B (DRPB) | Dynamin-Related Protein B (DRPB) | |
| Heat Shock Protein 90, putative |
ND |
ND |
ND |
|
| 100 kDa band | ND | ND | ND | FUSE-Binding Protein 2 / KH-type splicing regulatory protein (FUBP2) |
| DnaK family protein | ND | ND | ND | |
| Aconitate hydratase ACN/IRP | ND | Aconitate hydratase ACN/IRP | Aconitate hydratase ACN/IRP | |
| Cytosolic tRNA-Ala synthetase | Cytosolic tRNA-Ala synthetase | Cytosolic tRNA-Ala synthetase | Cytosolic tRNA-Ala synthetase | |
| Peptidase M16 inactive domain-containing protein | ND | ND | ND | |
| ND | Inner Membrane Complex protein 2A (IMC2A) | ND | ND | |
| Toxolysin 4 (TLN4) | Toxolysin 4 (TLN4) | Toxolysin 4 (TLN4) | ND | |
| ND | Ulp1 protease family, C-terminal catalytic domain-containing protein | ND | ND |
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