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Table 1
Proteins of Toxoplasma gondii RH strain identified by LC-MS/MS for the first IgM triplet spot of interest (spot 21) (ND: not detected).
| Protein name | Known or predicted function | Location | Accession | Organism | Molecular weight (Da) | Peptide count on protein profile No. 1 | Peptide count on protein profile No. 2 |
|---|---|---|---|---|---|---|---|
| Heat Shock Protein (HSP) | Molecular chaperone involved in protein folding, cellular stress responses, and regulation of host immune system | TGME49_chrXII:5582774-5587740(−) | TGME49_251780 | Toxoplasma_gondii_ME49 | 78251 | 56 | 35 |
| Heat Shock Protein 70 (HSP70) | Molecular chaperone involved in protein folding, cellular stress responses, and regulation of host immune system (modulation of nitric oxide production and reduction of macrophages phagocytosis capacity) | TGME49_chrVIII:3289396-3291420(−) | TGME49_273760 | Toxoplasma_gondii_ME49 | 72880 | 53 | 51 |
| Chaperonin protein BiP | Molecular chaperone involved in protein folding and exit from the endoplasmic reticulum | TGME49_chrXI:2225378-2228753(−) | TGME49_311720 | Toxoplasma_gondii_ME49 | 73253 | 43 | 26 |
| Heat Shock Protein 90 (HSP90) | Molecular chaperone involved in protein folding, cellular stress responses, and regulation of host immune system | TGME49_chrIX:2483404-2487798(+) | TGME49_288380 | Toxoplasma_gondii_ME49 | 81933 | 32 | 30 |
| Alveolin domain containing intermediate filament IMC1 (ALV1) | Inner membrane complex protein associated with the membrane cytoskeleton of Toxoplasma gondii, involved in maintaining parasite morphology and motility | TGME49_chrVIII:1516857-1519632(+) | TGME49_231640 | Toxoplasma_gondii_ME49 | 69596 | 25 | 14 |
| Peptidase M16 inactive domain-containing protein | Member of the M16A metalloprotease family, no function described | TGME49_chrX:716364-726488(+) | TGME49_227948 | Toxoplasma_gondii_ME49 | 142764 | 13 | 2 |
| Tryptophanyl-tRNA synthetase (TrpRS2) | Apicoplast protein involved in the aminoacylation of tRNA during proteins synthesis | TGME49_chrIX:2473710-2478137(+) | TGME49_288360 | Toxoplasma_gondii_ME49 | 77058 | 10 | 9 |
| Toxolysin 4 (TLN4) | Member of the M16A metalloprotease family, involved in Toxoplasma gondii egress from host cells and maturation of microneme proteins | TGME49_chrVIIa:903405-917753(+) | TGME49_206510 | Toxoplasma_gondii_ME49 | 246741 | 10 | 2 |
| Hypothetical protein | No function described | TGME49_chrVIIa:3523795-3527924(−) | TGME49_202200 | Toxoplasma_gondii_ME49 | 87043 | 10 | ND |
| Cytosolic tRNA-Ala synthetase | Cytosolic protein involved in the aminoacylation of tRNA during proteins synthesis | TGME49_chrXII:862344-876041(−) | TGME49_219540 | Toxoplasma_gondii_ME49 | 139516 | 7 | 4 |
| Vacuolar ATP synthase subunit A, putative | Proton-transporting ATP synthase activity contributing to endomembrane compartments acidification, organelle maturation, and intracellular pH regulation | TGME49_chrVIIb:4315661-4325274(−) | TGME49_256970 | Toxoplasma_gondii_ME49 | 68122 | 6 | 3 |
| Hypothetical protein | No function described | TGME49_chrVIII:2878595-2883425(−) | TGME49_233890 | Toxoplasma_gondii_ME49 | 73697 | 6 | 2 |
| Membrane Occupation and Recognition Nexus protein 2 (MORN2) | No function described, hypothetical role in cytoskeleton organization | TGME49_chrIX:4562922-4564319(+) | TGME49_292120 | Toxoplasma_gondii_ME49 | 49236 | 6 | ND |
| Hypothetical protein | No function described | TGME49_chrII:644118-650964(+) | TGME49_221870 | Toxoplasma_gondii_ME49 | 144135 | 6 | ND |
| Heat Shock Protein 90, putative | Molecular chaperone involved in protein folding, cellular stress responses, and regulation of host immune system | TGME49_chrVI:3356828-3362144(+) | TGME49_244560 | Toxoplasma_gondii_ME49 | 96823 | 5 | 1 |
| Aconitate hydratase ACN/IRP | Mitochondrial/apicoplast enzyme involved in the energy metabolism of Toxoplasma gondii (Krebs cycle) and regulation of host immune system (modulation of nitric oxide production) | TGME49_chrX:1400984-1408155(−) | TGME49_226730 | Toxoplasma_gondii_ME49 | 114885 | 5 | 1 |
| SAG-related sequence SRS29B (SRS29B) | Surface protein involved in host cell attachment | TGME49_chrVIII:2664481-2665491(+) | TGME49_233460 | Toxoplasma_gondii_ME49 | 34732 | 4 | 4 |
| DnaK family protein | ATP-dependent chaperone involved in protein folding | TGME49_chrX:1311834-1315968(−) | TGME49_226830 | Toxoplasma_gondii_ME49 | 103193 | 4 | 2 |
| Tetratricopeptide repeat-containing protein | ATP-dependent chaperone involved in protein folding | TGME49_chrIa:1252410-1260641(−) | TGME49_294898 | Toxoplasma_gondii_ME49 | 88633 | 4 | 2 |
| Actin 1 (ACT1) | Glideosome protein involved in gliding movement of Toxoplasma gondii, organelles distribution during cell division and secretory vesicles movement within the parasite | TGME49_chrIb:998462-1000024(+) | TGME49_209030 | Toxoplasma_gondii_ME49 | 41908 | 3 | 3 |
| Alpha Tubulin A1 (TUBA1) | Microtubule-associated protein involved in maintaining Toxoplasma gondii’s shape, intracellular transport, and apical complex organization | TGME49_chrXI:5274572-5276593(+) | TGME49_316400 | Toxoplasma_gondii_ME49 | 50114 | 3 | 2 |
| HEAT repeat-containing protein | Protein involved in intracellular transport, chromosome segregation, and signal transduction | TGME49_chrXI:6061226-6067819(−) | TGME49_216590 | Toxoplasma_gondii_ME49 | 111196 | 3 | ND |
| Hypothetical protein | No function described | TGME49_chrVIIa:2062064-2074174(+) | TGME49_203980 | Toxoplasma_gondii_ME49 | 201280 | 3 | ND |
| Chaperonin cpn60, putative | ATP-dependent chaperone involved in protein folding | TGME49_chrVI:1335457-1339036(+) | TGME49_240600 | Toxoplasma_gondii_ME49 | 72237 | 3 | ND |
| Protein disulfide isomerase | Endoplasmic reticulum enzyme involved in protein folding and redox homeostasis contributing to maturation and quality control of secretory and membrane proteins | TGME49_chrIV:1626551-1628375(+) | TGME49_211680 | Toxoplasma_gondii_ME49 | 52802 | 2 | 3 |
| Hypothetical protein | No function described | TGME49_chrXI:4609959-4617338(−) | TGME49_315270 | Toxoplasma_gondii_ME49 | 84600 | 2 | 2 |
| Histone lysine methyltransferase, SET, putative | Histone-lysine N-methyltransferase activity involved in epigenetic regulation | TGME49_chrVIIb:1042995-1044560(−) | TGME49_262750 | Toxoplasma_gondii_ME49 | 53633 | 2 | 1 |
| EMP24/GP25L/p24 family protein | Family of proteins involved in protein transport between endoplasmic reticulum, Golgi, and specialized organelles (micronemes or rhoptries) | TGME49_chrXI:1568699-1573498(+) | TGME49_310750 | Toxoplasma_gondii_ME49 | 64977 | 2 | 1 |
| D-3-phosphoglycerate dehydrogenase | Enzyme contributing to amino acid metabolism and providing precursors for nucleotide and lipid biosynthesis | TGME49_chrVI:939130-947919(−) | TGME49_239820 | Toxoplasma_gondii_ME49 | 65194 | 2 | ND |
| Cell division protein C48CY (CDC48CY) | AAA + ATPase protein involved in protein unfolding, degradation, membrane fusion, and regulation of turnover during cell cycle transitions | TGME49_chrVIII:3664539-3673736(−) | TGME49_273090 | Toxoplasma_gondii_ME49 | 89397 | 2 | ND |
| Microneme protein 1 (MIC1) | Protein secreted from apical micronemes during initial stages of host cell entry, forming a complex with MIC4 and MIC6 to mediate adhesion to host receptors | TGME49_chrIX:4395345-4397688(−) | TGME49_291890 | Toxoplasma_gondii_ME49 | 48629 | 1 | 2 |
| Inner Membrane Complex protein IMC2A (IMC2A) | Inner membrane complex protein contributing to the structural integrity and organization of the parasite pellicle | TGME49_chrX:520228-525722(−) | TGME49_228170 | Toxoplasma_gondii_ME49 | 170816 | ND | 11 |
| Cathepsin B | Cysteine protease localized to the endolysosomal system, involved in proteolytic processing, digestion of host macromolecules, and protein turnover | TGME49_chrXII:4721208-4726837(−) | TGME49_249670 | Toxoplasma_gondii_ME49 | 62477 | ND | 8 |
| Nuclear Factor 4 (NF4) | Putative transcription factor regulating gene expression | TGME49_chrVIIa:1293654-1297697(+) | TGME49_205580 | Toxoplasma_gondii_ME49 | 51221 | ND | 7 |
| Alveolin domain containing intermediate filament IMC4 (ALV4) | Inner membrane complex protein contributing to the cytoskeletal scaffold that provides mechanical strength and structural support to the parasite | TGME49_chrVIII:1513141-1515301(−) | TGME49_231630 | Toxoplasma_gondii_ME49 | 50172 | ND | 4 |
| Ubiquitin family protein | Regulator of protein turnover through the ubiquitin-proteasome system | TGME49_chrV:633282-639366(+) | TGME49_212820 | Toxoplasma_gondii_ME49 | 119003 | ND | 3 |
| Hypothetical protein | No function described | TGME49_chrXI:1880862-1900195(+) | TGME49_311230 | Toxoplasma_gondii_ME49 | 504758 | ND | 2 |
| AMP-binding enzyme domain-containing protein | Protein involved in metabolic pathways such as fatty acid activation, amino acid adenylation, and secondary metabolite biosynthesis, contributing to parasite energy metabolism and adaptation | TGME49_chrXI:1092557-1096957(−) | TGME49_310150 | Toxoplasma_gondii_ME49 | 95937 | ND | 2 |
| NTPase I | Enzyme involved in energy metabolism, signal transduction, and regulation of nucleotide hydrolysis-dependent cellular processes | TGME49_chrXII:6699033-6699642(−) | TGME49_277240 | Toxoplasma_gondii_ME49 | 22641 | ND | 2 |
| Dynamin-Related Protein B (DRPB) | Member of the dynamin superfamily of GTPases involved in membrane remodeling processes | TGME49_chrIb:1689989-1700172(+) | TGME49_321620 | Toxoplasma_gondii_ME49 | 95931 | ND | 2 |
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