Figure 2
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2D immunoblot of T. gondii RH strain proteins. A and B. Obtained after incubation with two NNTPos sera (patients 5BB (A) and 8BB (B)) and revelation with peroxidase-conjugated goat anti-human IgM antibodies. C and D. Obtained after incubation with two NNTNeg sera (patients 17BB (C) and 19BB (D)) and revelation with peroxidase-conjugated goat anti-human IgM antibodies. These immunoblots reveal the IgM triplet spots of interest (21–24) present only in NNTPos sera and identified by superposition with the protein profile of the T. gondii RH strain (black box). These immunoblots also make it possible to exclude the spots that are not specific to the IgM triplet (25 and 26) present in NNTPos and NNTNeg sera and identified by superposition with the protein profile of the T. gondii RH strain (NNTPos: newborns positive for the IgM triplet; NNTNeg: newborns negative for the IgM triplet).
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