Open Access

Table 1

Primer sequences targeting the SSU rRNA gene of Cryptosporidium sp.

Primer sequences (5′–3′) Target fragment size (bp) Annealing temperature (°C)
F1: GACATATCATTCAAGTTTCTGACC 763 58
R1: CTGAAGGAGTAAGGAACAACC
F2: CCTATCAGCTTTAGACGGTAGG 587 58
R2: TCTAAGAATTTCACCTCTGACTG
gp60F1: TTTACCCACACCATCTGTAGCGTCG 1044 58
gp60R1: ACGGACGGAATGATGTATCTGA
gp60F2: ATAGGTGATAATTAGTCAGTCTTTAAT 948 55
gp60R2: TCCAAAAGCGGCTGAGTCAGCATC

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