Open Access

Table 1

Experimental design for the evaluation of various factors and the daily feeding schedule for Aedes albopictus in Experiment 1. The standard regime used ground ingredients. Treatments with an (*) used the same trays.

Factors evaluated Description Diet preparation IAEA larval diet: quantity in mg per larva per day (volume in mL for 18,000 larvae)
Day 1 (Friday) Day 2 (Saturday) Day 3 (Sunday) Day 4 (Monday) Day 5 (Tuesday) Day 6 (Wednesday) Total First sorting time
Standard regime 4% (w/v) 0.11 (50) 0.22 (100) 0.44 (200) 0.44 (200) 0.33 (150) 0.11 (50) 1.65 (750) Day 6, 11 am (24 h)
Diet particle size Ground ingredients 6% (w/v) 0.23 (60) 0.23 (60) 0.83 (250) 0 1.26 (380) 1.26 (380) 3.81 (1130) Day 6, 11 am (24 h)
Unground ingredients 6% (w/v) 0.23 (60) 0.23 (60) 0.83 (250) 0 1.26 (380) 1.26 (380) 3.81 (1130) Day 6, 11 am (24 h)
Mosquito strain Rimini* strain 6% (w/v) 0.17 (50) 0.33 (100) 0.67 (200) 0.67 (200) 0.5 (150) 0.17 (50) 2.51 (750) Day 6, 11 am (24 h)
Guangzhou strain 6% (w/v) 0.17 (50) 0.33 (100) 0.67 (200) 0.67 (200) 0.5 (150) 0.17 (50) 2.51 (750) Day 6, 11 am (24 h)
Feeding regime Regime 1 6% (w/v) 0.22 (100) 0.44 (200) 0.88 (200 + 200) 0 0 0.44 (200) 1.98 (900) Day 6, 11 am (24 h)
Regime 2 6% (w/v) 0.23 (60) 0.23 (60) 0.83 (250) 0 1.26 (380) 1.26 (380) 3.81 (1130) Day 6, 11 am (24 h)
Regime 3* 6% (w/v) 0.17 (50) 0.33 (100) 0.67 (200) 0.67 (200) 0.5 (150) 0.17 (50) 2.51 (750) Day 6, 11 am (24 h)
Sex sorting time after onset pupation 24 h* 6% (w/v) 0.17 (50) 0.33 (100) 0.67 (200) 0.67 (200) 0.5 (150) 0.17 (50) 2.51 (750) Day 6, 11 am (24 h)
48 h 6% (w/v) 0.17 (50) 0.33 (100) 0.67 (200) 0.67 (200) 0.5 (150) 0.17 (50) 2.51 (750) Day 7, 11 am (48 h)

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