Table 2
PCR reagents in the order and concentration they were added.
| Reagents | Concentration of stock solution | Volume | Final concentration |
|---|---|---|---|
| Distilled water | – | 4 μL | – |
| Master mix | 2X | 10 μL | 1X |
| Forward primer | 10 μM = 10 pmols/μL | 1.0 μL | 0.5 pmols |
| Reverse primer | 10 μM = 10 pmols/μL | 1.0 μL | 0.5 pmols |
| Sample DNA | – | 4 μL | 20 ng/μL |
| Total (reaction) volume | – | 20 μL | – |
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