Open Access
Table 2
List of primers used for PCR amplification of nuclear markers in the present study.
| rDNA fragment | Primer name | Direction | Sequence (5′–3′) | PCR thermal profile | Product size | Reference |
|---|---|---|---|---|---|---|
| 18S + ITS1 | S1 | Forward | ATT CCG ATA ACG AAC GAG ACT | 94 °C:2 min; 39 × (94 °C: 60 s, 53 °C: 60 s, 72 °C: 90 s); 72 °C: 10 min | ~ 1000 bp | [52] |
| IR8 | Reverse | GCT AGC TGC GTT CTT CAT CGA | [55] | |||
| 28S | C1 | Forward | ACC CGC TGA ATT TAA GCA | 94 °C:2 min; 39 × (94 °C: 20 s, 56 °C: 30 s, 72 °C: 90 s); 72 °C: 10 min | ~ 800 bp | [19] |
| D2 | Reverse | TGG TCC GTG TTT CAA GAC | [19] |
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