Open Access
Table 1
Primers used in PCR analysis for Babesia species in skunks.
| Species | Region | Amplicon size | Primer name | Sequence (5′ 3″) | References |
|---|---|---|---|---|---|
| All Babesia primary | V4 of 18S | 500 bp | 5.1 | CCTGGTTGATCCTGCCAGTAGT | [20, 28] |
| B | TGATCCTTCTGCAGGTTCACCTAC | ||||
| All Babesia secondary | Babesia F | GTGAAACTGCGAATGGCTCA | [18] | ||
| Babesia R | CCATGCTGAAGTATTCAAGAC | ||||
| Babesia sensu stricto sp. | cox1 | 1080 bp | BabcoxF | GGAAGTGGWACWGGWTGGAC | [1] |
| BabcoxR | TTCGGTATTGCATGCCTTG | ||||
| Babesia microti-like sp. | cox1 | 600 | Cox1F133 | GGAGAGCTAGGTAGTAGTGGAGATAGG | [21] |
| Cox1R1130 | GTGGAAGTGAGCTACCACATACGCTG |
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