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Phylogenetic relationships among the Blastocystis sequences identified in this study were analyzed alongside previously stored sequences in GenBank, using the maximum likelihood method. This approach used the Tamura-3 parameter model within the Mega 7 software package. The percentages on the branches represent the bootstrap values obtained from 1,000 replicates. These sequences were uniquely identified by their accession number, host origin, and ST designation. The novel and known sequences identified in this study are marked with blue and black squares, respectively.

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